Lebendbeobachtungen und elektronenmikroskopische Untersuchungen der Neuentstehung von Mitochondrien in gezüchteten Hühnerherzzellen
Lebendbeobachtungen und elektronenmikroskopische Untersuchungen der Neuentstehung von Mitochondrien in gezüchteten Hühnerherzzellen
In irradiated and unirradiated chicken heart myoblasts cultivated in vitro, the formation of mitochondria was studied by means of phase contrast and electron microscopy. By phase contrast observations of' the living object it was found, that 6 to 7 hours after irradiation with 2400 R, the numbe...
| Personal Name(s): | Wendt, E. |
|---|---|
| Schäfer, D. | |
| Contributing Institute: |
Publikationen vor 2000; PRE-2000; Retrocat |
| Imprint: |
Jülich
Kernforschungsanlage Jülich, Verlag
1967
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| Physical Description: |
16 p. |
| Document Type: |
Report Book |
| Research Program: |
ohne Topic |
| Series Title: |
Berichte der Kernforschungsanlage Jülich
492 |
| Link: |
OpenAccess OpenAccess |
| Publikationsportal JuSER |
|
In irradiated and unirradiated chicken heart myoblasts cultivated in vitro, the formation of mitochondria was studied by means of phase contrast and electron microscopy. By phase contrast observations of' the living object it was found, that 6 to 7 hours after irradiation with 2400 R, the number of functionally active mitochondria decreased; subsequently there appeared in the cytoplasm numerous small spherical pre-mitochondria which soon increased in number and develop into mitochondria of normal size and structure. The results with the electron microscope are illustrated in the diagram (Plate 4). The endoplasmic reticulum produces buds which must be considered as young pre-mitochondria (a). While they are still attached to the reticular system (b$_{1}$) or after detaching from the endoplasmicreticulum (b$_{2}$) they begin to establish an interior membrane. Later on, in limited areas, the spherical pre-mitochondria show the typical arrangement of cristae mitochondriales (c) and simultanously they increase in length (d). Therewith the pre-mitochondria assume a club-shaped form (e) the spherical portion of which is filled with a dense ground-substance. This ground-substance obviously produces the material for the further formation of cristae mitochondriales which accompanies the outgrowth of the mitochondrial tube. Thus the pre-mitochondria transform into the definitive mitochondria (f). |