This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.1021/acs.bioconjchem.7b00645 in citations.
Biocatalytically Active Thin Films via Self-Assembly of 2-Deoxy- d -ribose-5-phosphate Aldolase–Poly( N -isopropylacrylamide) Conjugates
Biocatalytically Active Thin Films via Self-Assembly of 2-Deoxy- d -ribose-5-phosphate Aldolase–Poly( N -isopropylacrylamide) Conjugates
2-Deoxy-d-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substr...
Saved in:
Personal Name(s): | Zhang, Shuhao |
---|---|
Bisterfeld, Carolin / Bramski, Julia / Vanparijs, Nane / De Geest, Bruno G. / Pietruszka, Jörg / Böker, Alexander / Reinicke, Stefan (Corresponding author) | |
Contributing Institute: |
Institut für Bioorganische Chemie (HHUD); IBOC Biotechnologie; IBG-1 |
Published in: | Bioconjugate chemistry, 29 (2018) 1, S. 104-116 |
Imprint: |
Columbus, Ohio
American Chemical Society
2018
|
DOI: |
10.1021/acs.bioconjchem.7b00645 |
PubMed ID: |
29182313 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
2-Deoxy-d-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substrate as well as product inhibition requires a more-sophisticated process design for the synthesis of these motifs. One way to do so is to the couple aldehyde conversion with transport processes, which, in turn, would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. Consequently, we developed a fabrication process for such films that is based on the formation of DERA–poly(N-isopropylacrylamide) conjugates that are subsequently allowed to self-assemble at an air–water interface to yield the respective film. In this contribution, we discuss the conjugation conditions, investigate the interfacial properties of the conjugates, and, finally, demonstrate a successful film formation under the preservation of enzymatic activity. |