This title appears in the Scientific Report :
2018
Please use the identifier:
http://dx.doi.org/10.1016/j.copbio.2018.01.024 in citations.
Quantitative measurements in single-cell analysis: towards scalability in microbial bioprocess development
Quantitative measurements in single-cell analysis: towards scalability in microbial bioprocess development
Single-cell analysis in microfluidic cultivation devices bears a great potential for the development and optimization of industrial bioprocesses. High parallelization allows running a large number of cultivation experiments simultaneously even under quick alteration of environmental conditions. For...
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Personal Name(s): | Demling, Philipp (Corresponding author) |
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Westerwalbesloh, Christoph / Noack, Stephan / Wiechert, Wolfgang / Kohlheyer, Dietrich | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Current opinion in biotechnology, 54 (2018) S. 121 - 127 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2018
|
DOI: |
10.1016/j.copbio.2018.01.024 |
PubMed ID: |
29597183 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
Single-cell analysis in microfluidic cultivation devices bears a great potential for the development and optimization of industrial bioprocesses. High parallelization allows running a large number of cultivation experiments simultaneously even under quick alteration of environmental conditions. For example, the impact of changes in media composition on cell growth during classical batch cultivation can be easily resolved. A missing link for the scalability of microfluidic experiments is, however, their complete characterization via conventional performance indicators such as product titer and productivity. While existing mass spectrometry technology is not yet sufficiently coupled with microfluidics, optical methods like enzymatic assays or fluorescence sensors are promising alternatives but require further improvement to generate quantitative measurements of extracellular metabolites. |