This title appears in the Scientific Report :
2019
Please use the identifier:
http://hdl.handle.net/2128/21658 in citations.
Please use the identifier: http://dx.doi.org/10.1021/acs.analchem.8b03855 in citations.
Single-Molecule Techniques and Cell-Free Protein Synthesis: A Perfect Marriage
Single-Molecule Techniques and Cell-Free Protein Synthesis: A Perfect Marriage
Single-molecule techniques are nowadays an essential tool to study conformational changes as well as synthesis and folding of proteins. However, preparation of suitable protein samples is often time consuming and demanding. The rapid development of cell-free protein synthesis over the last years ope...
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Personal Name(s): | Katranidis, Alexandros (Corresponding author) |
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Fitter, Jörg (Corresponding author) | |
Contributing Institute: |
Molekulare Biophysik; ICS-5 |
Published in: | Analytical chemistry, 91 (2019) 4, S. 2570 - 2576 |
Imprint: |
Columbus, Ohio
American Chemical Society
2019
|
PubMed ID: |
30648382 |
DOI: |
10.1021/acs.analchem.8b03855 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Link: |
Restricted Restricted Published on 2019-01-16. Available in OpenAccess from 2020-01-16. Published on 2019-01-16. Available in OpenAccess from 2020-01-16. |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1021/acs.analchem.8b03855 in citations.
Single-molecule techniques are nowadays an essential tool to study conformational changes as well as synthesis and folding of proteins. However, preparation of suitable protein samples is often time consuming and demanding. The rapid development of cell-free protein synthesis over the last years opened new perspectives for fast and easy sample preparation but this was not fully exploited until now. Here, we take a look at the advancements in sample preparation as well as in the development of technical approaches and analytical tools, which unavoidably lead to the combination of single-molecule techniques and cell-free protein synthesis. It’s an ideal combination that can unlock the full potential of studying complex biological processes in the near future. |