This title appears in the Scientific Report :
2019
Please use the identifier:
http://dx.doi.org/10.1021/acs.jpcb.9b01239 in citations.
Please use the identifier: http://hdl.handle.net/2128/23521 in citations.
Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules
Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules
Effects of molecular crowding on structural and dynamical properties of biological macromolecules do depend on the concentration of crowding agents but also on the molecular mass and the structural compactness of the crowder molecules. By employing fluorescence correlation spectroscopy (FCS), we inv...
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Personal Name(s): | Junker, Niklas O. |
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Vaghefikia, Farzaneh / Albarghash, Alyazan / Höfig, Henning / Kempe, Daryan / Walter, Julia / Otten, Julia / Pohl, Martina / Katranidis, Alexandros / Wiegand, Simone / Fitter, Jörg (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 Weiche Materie; ICS-3 Molekulare Biophysik; ICS-5 |
Published in: |
The journal of physical chemistry |
Imprint: |
Washington, DC
Soc.66306
2019
|
DOI: |
10.1021/acs.jpcb.9b01239 |
PubMed ID: |
31059260 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Link: |
Restricted Restricted Published on 2019-05-06. Available in OpenAccess from 2020-05-06. Published on 2019-05-06. Available in OpenAccess from 2020-05-06. |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/23521 in citations.
Effects of molecular crowding on structural and dynamical properties of biological macromolecules do depend on the concentration of crowding agents but also on the molecular mass and the structural compactness of the crowder molecules. By employing fluorescence correlation spectroscopy (FCS), we investigated the translational mobility of several biological macromolecules ranging from 17 kDa to 2.7 MDa. Polyethylene glycol and Ficoll polymers of different molecular masses were used in buffer solutions to mimic a crowded environment. The reduction in translational mobility of the biological tracer molecules was analyzed as a function of crowder volume fractions and was generally more pronounced in PEG as compared to Ficoll solutions. For several crowding conditions, we observed a molecular sieving effect, in which the diffusion coefficient of larger tracer molecules is reduced to a larger extent than predicted by the Stokes–Einstein relation. By employing a FRET-based biosensor, we also showed that a multiprotein complex is significantly compacted in the presence of macromolecular crowders. Importantly, with respect to sensor in vivo applications, ligand concentration determining sensors would need a crowding specific calibration in order to deliver correct cytosolic ligand concentration. |