This title appears in the Scientific Report :
2019
Please use the identifier:
http://dx.doi.org/10.1002/adsc.201900183 in citations.
Condensing Enzymes from Pseudoalteromonadaceae for Prodiginine Synthesis
Condensing Enzymes from Pseudoalteromonadaceae for Prodiginine Synthesis
Prodiginines are natural products that are produced by various microorganisms. As the biological activity of prodiginines varies depending on their structure, prodiginine derivatives are of interest. In order to extend the prodiginine structural diversity, new condensing enzyme homologs of PigC were...
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Personal Name(s): | Brass, Hannah |
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Klein, Andreas / Nyholt, Silke / Classen, Thomas / Pietruszka, Jörg (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 Institut für Bioorganische Chemie (HHUD); IBOC |
Published in: | Advanced synthesis & catalysis, 361 (2019) 11, S. 2659-2667 |
Imprint: |
Weinheim
Wiley-VCH
2019
|
DOI: |
10.1002/adsc.201900183 |
Document Type: |
Journal Article |
Research Program: |
Innovative Synergisms |
Publikationsportal JuSER |
Prodiginines are natural products that are produced by various microorganisms. As the biological activity of prodiginines varies depending on their structure, prodiginine derivatives are of interest. In order to extend the prodiginine structural diversity, new condensing enzyme homologs of PigC were analysed. The condensing enzyme PigC from S. marcescens catalyses the last step in prodiginine biosynthesis and accepts not only its natural substrate, but also synthetically produced monopyrroles. Still, the substrate spectrum of PigC is partially limited. In order to enable the biocatalytic production of new prodiginine derivatives, PigC homologs supplementing the substrate spectra of PigC are of interest. Two condensing enzymes homologs from two tambjamine producing Pseudoalteromonadaceae strains were analysed towards their catalytic ability to produce the structurally related prodiginines. Besides the investigation of their substrate spectrum and enzyme kinetic data, a preparative scale biocatalysis approach for the production of prodiginine derivatives was established, comparing different condensing enzymes. |