This title appears in the Scientific Report : 2020 

Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance
Morschett, Holger
Jansen, Roman / Neuendorf, Christian / Moch, Matthias / Wiechert, Wolfgang / Oldiges, Marco (Corresponding author)
Biotechnologie; IBG-1
Journal of industrial microbiology & biotechnology, 47 (2020) S. 35-47
Berlin Springer67420 2020
10.1007/s10295-019-02243-w
Journal Article
Biotechnology
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OpenAccess
OpenAccess
Please use the identifier: http://hdl.handle.net/2128/24702 in citations.
Please use the identifier: http://dx.doi.org/10.1007/s10295-019-02243-w in citations.
Limited throughput represents a substantial drawback during bioprocess development. In recent years, several commercial microbioreactor systems have emerged featuring parallelized experimentation with optical monitoring. However, many devices remain limited to batch mode and do not represent the fed-batch strategy typically applied on an industrial scale. A workflow for 32-fold parallelized microscale cultivation of protein secreting Corynebacterium glutamicum in microtiter plates incorporating online monitoring, pH control and feeding was developed and validated. Critical interference of the essential media component protocatechuic acid with pH measurement was revealed, but was effectively resolved by 80% concentration reduction without affecting biological performance. Microfluidic pH control and feeding (pulsed, constant and exponential) were successfully implemented: Whereas pH control improved performance only slightly, feeding revealed a much higher optimization potential. Exponential feeding with µ = 0.1 h−1 resulted in the highest product titers. In contrast, other performance indicators such as biomass-specific or volumetric productivity resulted in different optimal feeding regimes.