This title appears in the Scientific Report :
2019
Please use the identifier:
http://dx.doi.org/10.1038/s42003-019-0709-7 in citations.
Please use the identifier: http://hdl.handle.net/2128/23599 in citations.
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we...
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Personal Name(s): | Höfig, Henning |
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Yukhnovets, Olessya / Remes, Cristina / Kempf, Noemie / Katranidis, Alexandros / Kempe, Daryan / Fitter, Jörg (Corresponding author) | |
Contributing Institute: |
Theorie der Weichen Materie und Biophysik; ICS-2 Molekulare Biophysik; ICS-5 |
Published in: | Communications biology, 2 (2019) 1, S. 459 |
Imprint: |
London
Springer Nature
2019
|
DOI: |
10.1038/s42003-019-0709-7 |
PubMed ID: |
31840104 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Link: |
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Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/23599 in citations.
Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria. |