This title appears in the Scientific Report :
2020
Please use the identifier:
http://dx.doi.org/10.1113/JP278658 in citations.
Please use the identifier: http://hdl.handle.net/2128/24403 in citations.
Relation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons
Relation between activity‐induced intracellular sodium transients and ATP dynamics in mouse hippocampal neurons
Excitatory neuronal activity results in the influx of Na+ through voltage‐ and ligand‐gated channels. Recovery from accompanying increases in intracellular Na+ concentrations ([Na+]i) is mainly mediated by the Na+/K+‐ATPase (NKA) and is one of the major energy‐consuming processes in the brain. Here,...
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Personal Name(s): | Gerkau, Niklas J. |
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Lerchundi, Rodrigo / Nelson, Joel S. E. / Lantermann, Marina / Meyer, Jan / Hirrlinger, Johannes / Rose, Christine R. (Corresponding author) | |
Contributing Institute: |
Zelluläre Biophysik; ICS-4 International Helmholtz Research School of Biophysics and Soft Matter; IHRS-BioSoft |
Published in: | The journal of physiology, 597 (2019) 23, S. 5687 - 5705 |
Imprint: |
Hoboken, NJ
Wiley-Blackwell
2019
|
PubMed ID: |
31549401 |
DOI: |
10.1113/JP278658 |
Document Type: |
Journal Article |
Research Program: |
International Helmholtz Research School of Biophysics and Soft Matter Physical Basis of Diseases |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/24403 in citations.
Excitatory neuronal activity results in the influx of Na+ through voltage‐ and ligand‐gated channels. Recovery from accompanying increases in intracellular Na+ concentrations ([Na+]i) is mainly mediated by the Na+/K+‐ATPase (NKA) and is one of the major energy‐consuming processes in the brain. Here, we analysed the relation between different patterns of activity‐induced [Na+]i signalling and ATP in mouse hippocampal CA1 pyramidal neurons by Na+ imaging with sodium‐binding benzofurane isophthalate (SBFI) and employing the genetically encoded nanosensor ATeam1.03YEMK (ATeam). In situ calibrations demonstrated a sigmoidal dependence of the ATeam Förster resonance energy transfer ratio on the intracellular ATP concentration ([ATP]i) with an apparent KD of 2.6 mm, indicating its suitability for [ATP]i measurement. Induction of recurrent network activity resulted in global [Na+]i oscillations with amplitudes of ∼10 mm, encompassing somata and dendrites. These were accompanied by a steady decline in [ATP]i by 0.3–0.4 mm in both compartments. Global [Na+]i transients, induced by afferent fibre stimulation or bath application of glutamate, caused delayed, transient decreases in [ATP]i as well. Brief focal glutamate application that evoked transient local Na+ influx into a dendrite, however, did not result in a measurable reduction in [ATP]i. Our results suggest that ATP consumption by the NKA following global [Na+]i transients temporarily overrides its availability, causing a decrease in [ATP]i. Locally restricted Na+ transients, however, do not result in detectable changes in local [ATP]i, suggesting that ATP production, together with rapid intracellular diffusion of both ATP and Na+ from and to unstimulated neighbouring regions, counteracts a local energy shortage under these conditions. |