This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/25257 in citations.
Please use the identifier: http://dx.doi.org/10.1007/s00253-020-10643-7 in citations.
Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis
Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis
Malonyl-CoA is an important central metabolite serving as the basic building block for the microbial synthesis of many pharmaceutically interesting polyketides, but also fatty acid–derived compounds including biofuels. Especially Saccharomyces cerevisiae, Escherichia coli, and Corynebacterium glutam...
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Personal Name(s): | Milke, Lars |
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Marienhagen, Jan (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Applied microbiology and biotechnology, 104 (2020) S. 6057–6065 |
Imprint: |
New York
Springer
2020
|
DOI: |
10.1007/s00253-020-10643-7 |
PubMed ID: |
32385515 |
Document Type: |
Journal Article |
Research Program: |
Innovative Synergisms |
Link: |
OpenAccess OpenAccess Restricted Restricted |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1007/s00253-020-10643-7 in citations.
Malonyl-CoA is an important central metabolite serving as the basic building block for the microbial synthesis of many pharmaceutically interesting polyketides, but also fatty acid–derived compounds including biofuels. Especially Saccharomyces cerevisiae, Escherichia coli, and Corynebacterium glutamicum have been engineered towards microbial synthesis of such compounds in recent years. However, developed strains and processes often suffer from insufficient productivity. Usually, tightly regulated intracellular malonyl-CoA availability is regarded as the decisive bottleneck limiting overall product formation. Therefore, metabolic engineering towards improved malonyl-CoA availability is essential to design efficient microbial cell factories for the production of polyketides and fatty acid derivatives. This review article summarizes metabolic engineering strategies to improve intracellular malonyl-CoA formation in industrially relevant microorganisms and its impact on productivity and product range, with a focus on polyketides and other malonyl-CoA-dependent products. |