This title appears in the Scientific Report :
2020
Please use the identifier:
http://hdl.handle.net/2128/25332 in citations.
Please use the identifier: http://dx.doi.org/10.1038/s41467-020-16503-2 in citations.
Distinct pre-initiation steps in human mitochondrial translation
Distinct pre-initiation steps in human mitochondrial translation
Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mitochondrial pre-initiation assembly steps involving those factors. Single-particle cryo-EM...
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Personal Name(s): | Khawaja, Anas |
---|---|
Itoh, Yuzuru / Remes, Cristina / Spåhr, Henrik / Yukhnovets, Olessya / Höfig, Henning / Amunts, Alexey (Corresponding author) / Rorbach, Joanna (Corresponding author) | |
Contributing Institute: |
Zelluläre Strukturbiologie; IBI-6 |
Published in: | Nature Communications, 11 (2020) 1, S. 2932 |
Imprint: |
[London]
Nature Publishing Group UK
2020
|
PubMed ID: |
32522994 |
DOI: |
10.1038/s41467-020-16503-2 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1038/s41467-020-16503-2 in citations.
Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mitochondrial pre-initiation assembly steps involving those factors. Single-particle cryo-EM revealed that in the first step, interactions between mitochondria-specific protein mS37 and mtIF3 keep the small mitoribosomal subunit in a conformation favorable for a subsequent accommodation of mtIF2 in the second step. Combination with fluorescence cross-correlation spectroscopy analyses suggests that mtIF3 promotes complex assembly without mRNA or initiator tRNA binding, where exclusion is achieved by the N-terminal and C-terminal domains of mtIF3. Finally, the association of large mitoribosomal subunit is required for initiator tRNA and leaderless mRNA recruitment to form a stable initiation complex. These data reveal fundamental aspects of mammal |