This title appears in the Scientific Report :
2020
Please use the identifier:
http://dx.doi.org/10.1038/s41467-020-18400-0 in citations.
Please use the identifier: http://hdl.handle.net/2128/25800 in citations.
Engineering and application of a biosensor with focused ligand specificity
Engineering and application of a biosensor with focused ligand specificity
Cell factories converting bio-based precursors to chemicals present an attractive avenue to a sustainable economy, yet screening of genetically diverse strain libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor. For this reason, transcriptional biosensors a...
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Personal Name(s): | Della Corte, Dennis |
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van Beek, Hugo / Syberg, Falk / Schallmey, Marcus / Tobola, Felix / Cormann, Kai / Schlicker, Christine / Baumann, Philipp Tobias / Krumbach, Karin / Sokolowsky, Sascha / Morris, Connor J. / Grünberger, Alexander / Hofmann, Eckhard / Schröder, Gunnar F. / Marienhagen, Jan (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 Strukturbiochemie; IBI-7 |
Published in: | Nature Communications, 11 (2020) 1, S. 4851 |
Imprint: |
[London]
Nature Publishing Group UK
2020
|
PubMed ID: |
32978386 |
DOI: |
10.1038/s41467-020-18400-0 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Link: |
Get full text OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/25800 in citations.
Cell factories converting bio-based precursors to chemicals present an attractive avenue to a sustainable economy, yet screening of genetically diverse strain libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor. For this reason, transcriptional biosensors attract attention as they allow the screening of vast libraries when used in combination with fluorescence-activated cell sorting (FACS). However, broad ligand specificity of transcriptional regulators (TRs) often prohibits the development of such ultra-high-throughput screens. Here, we solve the structure of the TR LysG of Corynebacterium glutamicum, which detects all three basic amino acids. Based on this information, we follow a semi-rational engineering approach using a FACS-based screening/counterscreening strategy to generate an l-lysine insensitive LysG-based biosensor. This biosensor can be used to isolate l-histidine-producing strains by FACS, showing that TR engineering towards a more focused ligand spectrum can expand the scope of application of such metabolite sensors. |