This title appears in the Scientific Report :
2022
Please use the identifier:
http://dx.doi.org/10.1093/plcell/koab265 in citations.
Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits
Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits
In plant mitochondria, nicotinamide adenine dinucleotide phosphate (NAD)-malic enzyme (NAD-ME) has a housekeeping function in malate respiration. In different plant lineages, NAD-ME was independently co-opted in C4 photosynthesis. In the C4 Cleome species Gynandropsis gynandra and Cleome angustifoli...
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Personal Name(s): | Hüdig, Meike |
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Tronconi, Marcos A (Corresponding author) / Zubimendi, Juan P / Sage, Tammy L / Poschmann, Gereon / Bickel, David / Gohlke, Holger / Maurino, Veronica G | |
Contributing Institute: |
Strukturbiochemie; IBI-7 John von Neumann - Institut für Computing; NIC Jülich Supercomputing Center; JSC Bioinformatik; IBG-4 |
Published in: | The plant cell, 34 (2022) 1, S. 597–615 |
Imprint: |
Rockville, Md.
Soc.
2022
|
DOI: |
10.1093/plcell/koab265 |
PubMed ID: |
34734993 |
Document Type: |
Journal Article |
Research Program: |
Molecular Information Processing in Cellular Systems Forschergruppe Gohlke Domain-Specific Simulation & Data Life Cycle Labs (SDLs) and Research Groups Utilization of renewable carbon and energy sources and engineering of ecosystem functions Biological and environmental resources for sustainable use |
Publikationsportal JuSER |
In plant mitochondria, nicotinamide adenine dinucleotide phosphate (NAD)-malic enzyme (NAD-ME) has a housekeeping function in malate respiration. In different plant lineages, NAD-ME was independently co-opted in C4 photosynthesis. In the C4 Cleome species Gynandropsis gynandra and Cleome angustifolia, all NAD-ME genes (NAD-MEα, NAD-MEβ1, and NAD-MEβ2) were affected by C4 evolution and are expressed at higher levels than their orthologs in the C3 species Tarenaya hassleriana. In Tarenaya hassleriana, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/β1 and NAD-MEα/β2, with similar biochemical properties. In both C4 species, this role is restricted to NAD-MEα/β2. In the C4 species, NAD-MEα/β1 is exclusively present in the leaves, where it accounts for most of the enzymatic activity. GgNAD-MEα/β1 exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate, confirming its role as the C4-decarboxylase. During C4 evolution, NAD-MEβ1 lost its catalytic activity; its contribution to the enzymatic activity results from a stabilizing effect on the associated α-subunit and the adquisition of regulatory properties. We conclude that in bundle sheath cell mitochondria of C4 species, the functions of NAD-ME as C4 photosynthetic decarboxylase and as a housekeeping enzyme coexist and are performed by isoforms that combine the same α subunit with differentially adapted β subunits. |