This title appears in the Scientific Report :
2022
Please use the identifier:
http://dx.doi.org/10.1016/j.str.2021.11.008 in citations.
Please use the identifier: http://hdl.handle.net/2128/31015 in citations.
The respiratory supercomplex from C. glutamicum
The respiratory supercomplex from C. glutamicum
Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIII2CIV2 supercomplex that catalyzes oxidation of menaquinol...
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Personal Name(s): | Moe, Agnes |
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Kovalova, Terezia / Król, Sylwia / Yanofsky, David J. / Bott, Michael / Sjöstrand, Dan / Rubinstein, John L. (Corresponding author) / Högbom, Martin / Brzezinski, Peter | |
Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | Structure, 30 (2022) 3, S. 338 - 349.e3 |
Imprint: |
Cambridge, Mass.
Cell Press
2022
|
DOI: |
10.1016/j.str.2021.11.008 |
PubMed ID: |
34910901 |
Document Type: |
Journal Article |
Research Program: |
Biological and environmental resources for sustainable use |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/31015 in citations.
Corynebacterium glutamicum is a preferentially aerobic gram-positive bacterium belonging to the phylum Actinobacteria, which also includes the pathogen Mycobacterium tuberculosis. In these bacteria, respiratory complexes III and IV form a CIII2CIV2 supercomplex that catalyzes oxidation of menaquinol and reduction of dioxygen to water. We isolated the C. glutamicum supercomplex and used cryo-EM to determine its structure at 2.9 Å resolution. The structure shows a central CIII2 dimer flanked by a CIV on two sides. A menaquinone is bound in each of the QN and QP sites in each CIII and an additional menaquinone is positioned ∼14 Å from heme bL. A di-heme cyt. cc subunit electronically connects each CIII with an adjacent CIV, with the Rieske iron-sulfur protein positioned with the iron near heme bL. Multiple subunits interact to form a convoluted sub-structure at the cytoplasmic side of the supercomplex, which defines a path for proton transfer into CIV. |