This title appears in the Scientific Report :
2022
Please use the identifier:
http://dx.doi.org/10.1093/jxb/erac184 in citations.
Please use the identifier: http://hdl.handle.net/2128/34227 in citations.
N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon
N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon
Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depend...
Saved in:
Personal Name(s): | Kuang, Weiqi |
---|---|
Sanow, Stefan / Kelm, Jana M / Müller Linow, Mark / Andeer, Peter / Kohlheyer, Dietrich / Northen, Trent / Vogel, John P / Watt, Michelle / Arsova, Borjana (Corresponding author) | |
Contributing Institute: |
Biotechnologie; IBG-1 Pflanzenwissenschaften; IBG-2 |
Published in: | The journal of experimental botany, 73 (2022) 15, S. 5306–5321 |
Imprint: |
Oxford
Oxford Univ. Press
2022
|
DOI: |
10.1093/jxb/erac184 |
Document Type: |
Journal Article |
Research Program: |
Biological and environmental resources for sustainable use |
Subject (ZB): | |
Link: |
Get full text OpenAccess Restricted Restricted OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/34227 in citations.
Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25–30 plants h−1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH4NO3 the benefit appears through N fixation, while at 0.5 mM NH4NO3 the mechanism appears to be plant physiological, with H. seropedicae promoting uptake of N from the root medium.Future work could fine-tune plant and root-associated microorganisms to growth and nutrient dynamics. |