This title appears in the Scientific Report :
2022
Please use the identifier:
http://hdl.handle.net/2128/31516 in citations.
Please use the identifier: http://dx.doi.org/10.1016/j.crchbi.2022.100031 in citations.
Enzyme co-localisation: Mechanisms and benefits
Enzyme co-localisation: Mechanisms and benefits
Co-localising enzymes can drastically affect their properties, such as stability, specificity, and activity, thus influencing reaction kinetics. In this review, we present a brief overview of the main methods developed for enzyme immobilisation, co-localisation, and conjugation and discuss how and w...
Saved in:
Personal Name(s): | Kondrat, Svyatoslav (Corresponding author) |
---|---|
Krauss, Ulrich / von Lieres, Eric | |
Contributing Institute: |
Biotechnologie; IBG-1 Institut für Molekulare Enzymtechnologie (HHUD); IMET |
Published in: | Current research in chemical biology, 2 (2022) S. 100031 - |
Imprint: |
[Amsterdam]
Elsevier B.V.
2022
|
DOI: |
10.1016/j.crchbi.2022.100031 |
Document Type: |
Journal Article |
Research Program: |
Biological and environmental resources for sustainable use |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1016/j.crchbi.2022.100031 in citations.
Co-localising enzymes can drastically affect their properties, such as stability, specificity, and activity, thus influencing reaction kinetics. In this review, we present a brief overview of the main methods developed for enzyme immobilisation, co-localisation, and conjugation and discuss how and why they affect the enzyme properties. We also describe the effects emerging from bringing two sequential enzymes of a cascade reaction together, particularly if and when it speeds up reaction velocity. Furthermore, we discuss enzyme compartmentalisation, or clustering of several enzymes of a cascade, and present theoretical approaches developed to optimise synthetic enzyme clusters. We also point out the plenitude of open questions, which exist despite the enormous research effort channelled into understanding enzyme co-localisation. |