Description: Dataset containing physiological amounts of spike-in proteins into murine C2C12 background as a ground truth quantitative LC-MS/MS reference

This title appears in the Scientific Report : 2022

Dataset containing physiological amounts of spike-in proteins into murine C2C12 background as a ground truth quantitative LC-MS/MS reference

In this article, we present a data dependent acquisition (DDA) dataset which was generated as a reference and ground truth quantitative dataset. While initially used to compare samples measured with DDA and data independent acquisition (DIA) (Barkovits et al., 2020), the presented dataset holds pote...

Personal Name(s):	Uszkoreit, Julian (Corresponding author)
	Barkovits, Katalin / Pacharra, Sandra / Pfeiffer, Kathy / Steinbach, Simone / Marcus, Katrin / Eisenacher, Martin
Contributing Institute:	Computergestütze Metagenomik; IBG-5
Published in:	Data in Brief, 43 (2022) S. 108435 -
Imprint:	Amsterdam [u.a.] Elsevier 2022
DOI:	10.1016/j.dib.2022.108435
Document Type:	Journal Article
Research Program:	ohne Topic
Link:	OpenAccess
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1016/j.dib.2022.108435 in citations.
Please use the identifier: http://hdl.handle.net/2128/33804 in citations.

In this article, we present a data dependent acquisition (DDA) dataset which was generated as a reference and ground truth quantitative dataset. While initially used to compare samples measured with DDA and data independent acquisition (DIA) (Barkovits et al., 2020), the presented dataset holds potential value as a benchmark reference for any workflows working on DDA data. The entire dataset consists of 15 LC-MS/MS measurements composed of five distinct spike-in-states, each with three replicates. To generate the data set, a C2C12 (immortalized mouse myoblast) cell lysate was used as a complex background for five different states which were simulated by spiking 13 defined proteins at different concentrations. For this purpose, the cell lysate was used in a constant amount of 20 µg for all samples and different amounts of the 13 selected proteins ranging from 0.1 to 10 pmol were added, reflecting physiological amounts of proteins. Afterwards, all samples were tryptically digested using the same method. From each sample 200 ng tryptic peptides were measured in triplicates on a Q Exactive HF (Thermo Fisher Scientific). The mass range for MS1 was set to 350–1400 m/z with a resolution of 60,000 at 200 m/z. HCD fragmentation of the Top10 abundant precursor ions was performed at 27% NCE. The fragment analysis (MS2) was performed with a resolution of 30,000 at 200 m/z.