High Performance Computing for Drug Discovery and Biomedicine [E-Book] /
Table of Contents: ... for Drug Discovery -- High Throughput Structure-Based Drug Design (HT-SBDD) Using Drug Docking, Fragment Molecular Orbital Calculations, and Molecular Dynamic Techniques -- HPC Framework for Performing In Silico Trials Using a 3D Virtual Human Cardiac Population...

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Food Allergens [E-Book] : Methods and Protocols /
Table of Contents: ... for Clinical Oral Challenges -- Structural Characterization of Food Allergens by Nuclear Magnetic Resonance Spectroscopy -- Co-Culture of Human Dendritic and T Cells for the Study of Specific T Cell-Mediated Responses against Food Allergens -- Evaluation...

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Vascular Hyperpermeability [E-Book] : Methods and Protocols /
Table of Contents: ... of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells -- Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs) -- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification -- Measurement of Blood-Brain...

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Deadenylation [E-Book] : Methods and Protocols /
Table of Contents: ...The reconstitution of human CCR4-NOT from purified proteins and an assay of its deadenylation activity -- In vitro reconstitution of the Drosophila melanogaster CCR4-NOT complex to assay deadenylation -- Analysis of human mRNA deadenylation complexes via...

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Cilia [E-Book] : Methods and Protocols /
Table of Contents: ...Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium -- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures -- A...

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Stem Cells and Lineage Commitment [E-Book] : Methods and Protocols /
Table of Contents: ... GapmeRs in Differentiating Human Induced Pluripotent Stem Cells (hiPSC) Towards Pancreatic Progenitors -- Retinoic Acid-Mediated Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells -- Isolation and Functional Analysis of Myoepithelial Cells from...

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Bacteriophages [E-Book] : Methods and Protocols /
Table of Contents: ...Structural and genomic diversity of bacteriophages -- The diversity of bacteriophages in human gut -- Breaking the ice - A review of phages from polar ecosystems -- The diversity of bacteriophages in hot spring -- Isolation of bacteriophage from soil...

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Psychophysiology Methods [E-Book] /
Tissue-Resident Macrophages [E-Book] : Methods and Protocols /
Table of Contents: ... Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell...

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Zebrafish [E-Book] : Methods and Protocols /
Positron Emission Tomography [E-Book] : Methods and Protocols /
Gene, Drug, and Tissue Engineering [E-Book] /
Table of Contents: ...-Mediated DNA Editing in Multicellular Eukaryotes -- Design of SaCas9-HF for In Vivo Gene Therapy -- Stem Cell Technology in Organ Transplantation: A Novel Method for 3D Bioprinting Functional and Stable Liver Grafts Using Human iPS-Derived Cells -- In Vitro...

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Protein Aggregation [E-Book] : Methods and Protocols /
Table of Contents: ... within human reconstructed neuronal network and associated neuronal dysfunctions -- Single-particle analysis of the interaction between molecules and protein aggregated species by Dual-Color Time-Resolved Fluorescence Spectroscopy -- FRAP & FRET investigation...

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West Nile Virus [E-Book] : Methods and Protocols  /
Haplotyping [E-Book] : Methods and Protocols  /
Table of Contents: ...Interrogating the human diplome: computational methods, emerging applications, and challenges -- Targeted Locus Amplification and Haplotyping -- Full length transcript phasing with third generation sequencing -- Large scale complete sequencing...

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Computer-Aided Antibody Design [E-Book] /
Table of Contents: ... simulation for investigating antigen-antibody interaction -- Molecular Dynamics Methods for Antibody Design -- Probing antibody dynamics with geometric simulations -- PITHA: a webtool to predict immunogenicity for humanized and fully human therapeutic antibodies...

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CCN Proteins [E-Book] : Methods and Protocols  /
Table of Contents: ... Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using...

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SILAC [E-Book] : Methods and Protocols  /
Table of Contents: ... and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons -- Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry -- SILAC-Based Quantitative...

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T-Cell Development [E-Book] : Methods and Protocols  /
Table of Contents: ... marrow precursors to T cells and ILCs -- Studying T cell Development in Neonatal and Adult Thymic Slices -- Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines -- Methods for Study of Mouse T Cell Receptor a and b...

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Difference Gel Electrophoresis [E-Book] : Methods and Protocols  /
Table of Contents: ...-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis -- 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens -- Identification of Subproteomic Markers for Skeletal Muscle Profiling -- DIGE Analysis of Fish Tissues -- Sample...

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