Reverse Genetics of RNA Viruses [E-Book] : Methods and Protocols /
Table of Contents: ... Genetics -- Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2 -- Construction of Infectious Clones for Human Enteroviruses -- Reverse genetics of Hepatitis C Virus using an RNA Polymerase I-Mediated Transcription -- Reverse Genetics...

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Pseudomonas aeruginosa [E-Book] : Methods and Protocols /
Table of Contents: ... fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs -- The Pseudomonas aeruginosa resistome: permanent and transient antibiotic resistance, an overview -- Biosensors for inducers of transient antibiotic resistance -- Pseudomonas...

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Deadenylation [E-Book] : Methods and Protocols /
Table of Contents: ...The reconstitution of human CCR4-NOT from purified proteins and an assay of its deadenylation activity -- In vitro reconstitution of the Drosophila melanogaster CCR4-NOT complex to assay deadenylation -- Analysis of human mRNA deadenylation complexes via...

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Zebrafish [E-Book] : Methods and Protocols /
Table of Contents: ... in Zebrafish -- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression -- Scalable CRISPR Screens in Zebrafish Using MIC-Drop -- The Goldfish Genome and Its Utility for Understanding Gene Regulation...

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Viral Metagenomics [E-Book] : Methods and Protocols /
Table of Contents: ... using ExTaxsI: The Monkeypox Use Case -- Optimized Recovery of Viral DNA and RNA from Blood Plasma for Viral Metagenomics -- Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches -- Viromes...

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Single Cell Analysis [E-Book] : Methods and Protocols /
Table of Contents: ... -- Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell -- Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients -- Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor Cells...

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Genomics Data Analysis for Crop Improvement [E-Book] /
Plant-Virus Interactions [E-Book] /
Table of Contents: ...Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing -- Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons -- Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral...

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Tissue-Resident Macrophages [E-Book] : Methods and Protocols /
Table of Contents: ...Macrophage Development and Function -- Fate Mapping Macrophages: From Ontogeny to Functions -- Studying Autophagy in Microglia: Overcoming the Obstacles -- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq -- Isolation of Tissue...

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Proteases and Cancer [E-Book] : Methods and Protocols /
Table of Contents: ... Levels by Enzyme-Linked Immunosorbent Assay (ELISA) -- Methods to investigate thrombin cleavage of osteopontin (OPN) -- Using siRNA silencing to analyze ADAM17 in macrophages -- Preparation and characterization of collagen-hyaluronic acid (Col-HA) matrices...

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Bacterial Regulatory RNA [E-Book] : Methods and Protocols /
Table of Contents: ...RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method -- Extraction and purification of outer membrane vesicles and their associated RNAs -- Analysis of phage regulatory RNAs: sequencing library...

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Rheumatoid Arthritis [E-Book] : Methods and Protocols /
Table of Contents: ... Docking Calculations -- RNA interference ex vivo -- Lentiviral-mediated systemic RNA interference in vivo -- Lentiviral production platform -- Mesenchymal stem cell engineering -- Screening of Ca2+ influx in lymphocytes -- Single-cell Ca2+ imaging...

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Teratogenicity Testing [E-Book] : Methods and Protocols /
Table of Contents: ... Technique in Teratogenicity Testing Using Zebrafish (Danio rerio) Embryos as Model -- Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos -- Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos -- Microarray Analysis...

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Thermomorphogenesis [E-Book] : Methods and Protocols /
Chen, Meng
Table of Contents: ... Electrophoretic Mobility Shift Assays -- Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism -- Characterization of PIF4 Phosphorylation by SPA1 -- Chromatin Immunoprecipitation to Investigate...

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Cerebral Cortex Development [E-Book] : Methods and Protocols /
Table of Contents: ... of Neural Circuits Governing Behavioral Plasticity in the Nematode Caenorhabditis elegans -- Social Behavior in Animal Models of Autism Spectrum Disorder -- Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq....

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Machine Learning Methods for Multi-Omics Data Integration [E-Book] /
Table of Contents: ... Yagin -- Chapter 4: Multimodal methods for knowledge discovery from bulk and single-cell multi-omics data, Yue Li, Gregory Fonseca, and Jun Ding -- Chapter 5: Negative sample selection for miRNA-disease association prediction models, Yulian Ding, Fei Wang...

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Proceedings of the 1st International Conference on Fluid, Thermal and Energy Systems [E-Book] : ICFTES 2022 /
Das, Sudev
Table of Contents: ...: A Computational Approach -- Enhancement Of Energy Storage Using Phase Change Material And Nano Materials In Advancement -- Numerical Study Of A Microchannel Thermocycler With Sequential Pcm Array For Rna Amplification -- Experimental And Numerical Studies...

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Advanced Materials for Multidisciplinary Applications [E-Book] /
Gao, Wei
Table of Contents: ...Leadership and Resiliency in the Global Chemistry Enterprise -- Chinese American Chemical Society (CACS): Overview and 40th Anniversary -- Universal vaccine development through glycoengineering -- Advances in Lipid Nanoparticles for mRNA Delivery: from...

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Double-Stranded RNA [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation -- Detection of dsRNA by Acridine Orange Staining -- Isolation of dsRNA from Plants by Cellulose Chromatography -- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column...

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Embryo Models In Vitro [E-Book] : Methods and Protocols /
Table of Contents: ... to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts -- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast -- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos -- Evaluation...

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