Table of Contents: “...Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo -- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System -- Efficient Depletion...”

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Table of Contents: “... Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu....”

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Table of Contents: “... -- Studying TDP1 Function in DNA Repair -- Topoisomerase II Chromatin Immunoprecipitation -- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation -- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae...”

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Table of Contents: “... and Their Effects on Biofilm Formation and Gene Expression -- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth -- Detection of Bacterial Quorum Sensing Molecules -- Generating Chromosome...”

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Table of Contents: “... of the Mitotic Exit Network at Spindle Pole Bodies -- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays -- Analysis of the Functionality of the Mitotic Checkpoints -- Cdc14 and Chromosome Condensation...”

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Table of Contents: “...Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells -- Assessment of HDACi-Induced Protein Cleavage by Caspases -- Assessment of HDACi-Induced Cytotoxicity -- Analysis of HDACi-Induced Changes in Chromosomal Passenger...”

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