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DNA Modifications [E-Book] : Methods and Protocols /
Table of Contents: ... -- Computational Analysis of DNA Modifications in Confocal Images -- Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei -- Analysis of 5-Hydroxymethyluracil Levels Using Flow Cytometry -- Hairpin-Bisulfite...

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Capturing Chromosome Conformation [E-Book] : Methods and Protocols  /
Table of Contents: ...Capturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome...

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Parasite Genomics [E-Book] : Methods and Protocols  /
Table of Contents: ...Nanopore Long Read DNA Sequencing of Protozoan Parasites: Hybrid Genome Assembly of Trypanosoma cruzi -- Chromosomes Conformation Capture Coupled with Next Generation Sequencing (Hi-C) in Plasmodium falciparum -- Sequencing and Reconstructing Helminth...

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Homologous Recombination [E-Book] : Methods and Protocols  /
Table of Contents: ... Electrophoresis and Southern Blotting -- Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe -- Quantifying DNA End Resection in Human Cells -- Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure...

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Epigenetic Reprogramming During Mouse Embryogenesis [E-Book] : Methods and Protocols /
Table of Contents: ... -- Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization -- Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development -- Deciphering the Early Mouse...

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Doubled Haploid Technology. Volume 1. General Topics, Alliaceae, Cereals [E-Book] /
Table of Contents: ... -- Methods for Chromosome Doubling -- Doubled Haploid Onion (Allium cepa L.) Production via In Vitro Gynogenesis -- In Vitro Gynogenesis in Leek (Allium ampeloprasum L.) -- Barley Isolated Microspore Culture -- Site-Directed Mutagenesis in Barley using RNA-GUided...

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MERS Coronavirus [E-Book] : Methods and Protocols  /
Table of Contents: ... of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen...

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Plant Meiosis [E-Book] : Methods and Protocols  /
Table of Contents: ...A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis...

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Cereal Genomics [E-Book] : Methods and Protocols /
Table of Contents: ... -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR...

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DNA Electrophoresis [E-Book] : Methods and Protocols  /
Table of Contents: ... Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection...

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DNA Repair [E-Book] : Methods and Protocols /
Table of Contents: ...-Fluorescence In Situ Hybridization (IF-FISH) -- FISHing for Damage on Metaphase Chromosomes....

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Imaging Gene Expression [E-Book] : Methods and Protocols /
Table of Contents: ... -- Detection of mRNAs Anchored to the Nuclear Envelope during Export Inhibition in Living Cells -- Programmable Chromosome Painting with Oligopaints -- Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices -- Targeted Manipulation...

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SMC Complexes : Methods and Protocols [E-Book] /
Table of Contents: ...Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo -- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System -- Efficient Depletion...

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Genome Instability [E-Book] : Methods and Protocols /
Table of Contents: ...The A-like Faker Assay for Measuring Yeast Chromosome III Stability -- The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast -- Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay -- Molecular Genetic...

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Erythropoiesis [E-Book] : Methods and Protocols /
Table of Contents: ... of Erythroblast Enucleation -- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure -- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples -- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples -- Genome Editing...

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DNA Methylation Protocols [E-Book] /
Table of Contents: ... Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual Chromosomes.-Helper-Dependent Chain Reaction (HDCR) for Selective Amplification of Methylated DNA Sequences.-DNA Methylation Analysis from Blood Spots: Increasing Yield...

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Next Generation Sequencing [E-Book] : Methods and Protocols /
Table of Contents: ... Analysis of RNA-Seq from Tissue Admixtures -- Next-Generation Sequencing of Genome-wide CRISPR Screens -- Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells -- Investigate Global Chromosomal Interaction by Hi-C in Human Naïve CD4...

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Chromothripsis [E-Book] : Methods and Protocols /
Table of Contents: ...The Genomic Characteristics and Origin of Chromothripsis -- Clinical Consequences of Chromothripsis and Other Catastrophic Cellular Events -- Potential Role of Chromothripsis in the Genesis of Complex Chromosomal Rearrangements in Human Gametes...

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CpG Islands [E-Book] : Methods and Protocols /
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Host-Pathogen Interactions [E-Book] : Methods and Protocols /
Table of Contents: ... and Their Effects on Biofilm Formation and Gene Expression -- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth -- Detection of Bacterial Quorum Sensing Molecules -- Generating Chromosome...

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