Table of Contents: “... and rice -- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize -- Genetic and phenotypic analyses of carpel development in Arabidopsis -- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis...”

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Table of Contents: “...Methods to Visualize the Actin Cytoskeleton during Plant Cell Division -- Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana -- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips -- Assaying Cyclin-Dependent...”

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Table of Contents: “... -- Interrogating global chromatin interaction network by high-throughput chromosome conformation capture (Hi-C) in plants -- Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat -- Super-resolution chromatin visualisation by combined fluorescence...”

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Table of Contents: “... -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR...”

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Table of Contents: “...A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis...”

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Table of Contents: “...Plant DNA Repair Pathways and their Applications in Genome Editing -- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method -- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations -- Creating Large Chromosomal...”

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Table of Contents: “... -- Illuminating Phytophthora Biology with Fluorescent Protein Tags -- Methods for Transient Gene Expression in Wild Relatives of Potato -- Host-induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat -- From Short Reads to Chromosome-scale Genome...”

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Table of Contents: “... -- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity -- Technical Review: A Hitchhikers Guide to Chromosome Conformation Capture -- 3C in Maize and Arabidopsis -- Profiling Histone Modifications in Synchronised Floral...”

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Table of Contents: “... Lines for QTL Mapping -- Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing -- Circular Chromosome Conformation Capture in Plants -- Genome Wide Profiling of Histone Modifications and Histone Variants...”

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Table of Contents: “... Spreading and Image Quality for the Study of Chromosomes in Plant Tissues -- Whole Mount RNA-FISH on Ovules and Developing Seeds -- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy -- Transmission Electron...”

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Table of Contents: “... with Cell Cycle Inhibitors -- Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry -- Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes -- Fluorescence In Situ Hybridization On Rice Chromosomes -- Dynamics...”

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Table of Contents: “...C-Banding of Plant Chromosomes -- Chromosome Painting by GISH and Multi-Color FISH -- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes -- Tyramide Signal Amplification: Fluorescence In Situ...”

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Table of Contents: “... with In Vivo Recombinase Mediated Gene Stacking -- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants -- One-Step Generation of Chromosomal Rearrangements in Rice -- Genome Elimination by Tailswap CenH3: In Vivo Haploid...”

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Table of Contents: “... Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice -- Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana -- Genetic and Phenotypic Analyses of Carpel Development...”

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Table of Contents: “... Developing or Mature Wheat Seeds -- cDNA Library Preparation -- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants -- The Polymerase Chain Reaction (PCR) - General Methods -- Mutation and Mutation Screening...”

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Table of Contents: “...The Model Legume Genomes -- Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization -- Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes -- RNA-Seq for Transcriptome Analysis...”

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Table of Contents: “... -- Phytoplasma Plasmid DNA Extraction -- Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA -- Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants -- Mapping the Phytoplasma Chromosome.....”

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Table of Contents: “...Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis -- Analysis of Plant Meiotic Chromosomes by Chromosome Painting -- Using Sequential Fluorescence...”

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