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Next Generation Sequencing [E-Book] : Methods and Protocols /
Table of Contents: ... Analysis of RNA-Seq from Tissue Admixtures -- Next-Generation Sequencing of Genome-wide CRISPR Screens -- Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells -- Investigate Global Chromosomal Interaction by Hi-C in Human Naïve CD4...

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Chromothripsis [E-Book] : Methods and Protocols /
Table of Contents: ...The Genomic Characteristics and Origin of Chromothripsis -- Clinical Consequences of Chromothripsis and Other Catastrophic Cellular Events -- Potential Role of Chromothripsis in the Genesis of Complex Chromosomal Rearrangements in Human Gametes...

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Urothelial Carcinoma [E-Book] : Methods and Protocols /
Table of Contents: ...Analysis of Chromosomal Alterations in Urothelial Carcinoma -- Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma -- A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer -- Immunohistochemical Analysis of UC...

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DNA Topoisomerases [E-Book] : Methods and Protocols /
Table of Contents: ... -- Studying TDP1 Function in DNA Repair -- Topoisomerase II Chromatin Immunoprecipitation -- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation -- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae...

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Genome Instability [E-Book] : Methods and Protocols /
Table of Contents: ...The A-like Faker Assay for Measuring Yeast Chromosome III Stability -- The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast -- Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay -- Molecular Genetic...

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DNA Methylation Protocols [E-Book] /
Table of Contents: ... Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual Chromosomes.-Helper-Dependent Chain Reaction (HDCR) for Selective Amplification of Methylated DNA Sequences.-DNA Methylation Analysis from Blood Spots: Increasing Yield...

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CpG Islands [E-Book] : Methods and Protocols /
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Meiosis [E-Book] /
Table of Contents: ... Recombination in Neurospora -- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae -- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast -- In Vivo...

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Haplotyping [E-Book] : Methods and Protocols /
Table of Contents: ... using Induced Aneuploidies and SNP/CGH Microarray Analysis -- Whole-genome Haplotyping of Single Sperm of Daphnia Pulex (Crustacea, Anomopoda) -- Chromosome-range Whole-genome High-throughput Experimental Haplotyping by single-chromosome Microdissection...

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Population Epigenetics [E-Book] : Methods and Protocols /
Table of Contents: ... to Identifying Genome Functionality Through Database Analysis -- Detecting Spatial Chromatin Organization by Chromosome Conformation Capture II: Genome-Wide Profiling by Hi-C -- Quantitative Analysis of Intra-chromosomal Contacts: The 3C-qPCR Method -- 5...

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Molecular Profiling [E-Book] : Methods and Protocols /
Table of Contents: ... Hybridization of Cells, Chromosomes, and Formalin Fixed Paraffin Embedded Tissues -- High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization -- Mass Spectrometry Based Biomarker Discovery -- Quantitative Mass...

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Innate Antiviral Immunity [E-Book] : Methods and Protocols /
Table of Contents: ... -- Chromosome Conformation Capture for Research on Innate Antiviral Immunity -- Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing -- Isolation, Purification, and Culture of Primary Murine Sensory Neurons -- Isolation...

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Enhancer RNAs [E-Book] : Methods and Protocols /
Table of Contents: ...) Protocol in Neurons -- Mapping Long non-coding RNA Chromatin Occupancy using Capture Hybridization Analysis of RNA Targets (CHART) -- Detecting long-range Enhancer-promoter Interactions by Quantitative Chromosome Conformation Capture -- Deciphering Noncoding RNA...

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Systems Genetics [E-Book] : Methods and Protocols /
Table of Contents: ... Regulating Complex Phenotypes -- RNA-Seq in the Collaborative Cross -- QTL Mapping and Identification of Candidate Genes in DO Mice: A Use Case Model Derived from a Benzene Toxicity Experiment -- Visualization of Results from System Genetics Studies in Chromosomal...

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Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation [E-Book] /
Table of Contents: ... Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq) -- Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells -- In vitro assay to Study Histone...

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Cancer Cytogenetics [E-Book] : Methods and Protocols /
Table of Contents: ...Cancer Cytogenetics: An Introduction -- Chromosome Preparation for Myeloid Malignancies -- Chromosome Preparation for Acute Lymphoblastic Leukemia -- Chromosome Preparation for Chronic Lymphoid Malignancies -- Cytogenetic Harvesting of Cancer Cells...

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Imaging Flow Cytometry [E-Book] : Methods and Protocols /
Table of Contents: ... Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry -- Quantitation of Chromosome Damage by Imaging Flow Cytometry -- Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry -- Analysis of Nucleo-Cytoplasmic Protein...

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Forensic DNA Typing Protocols [E-Book] /
Table of Contents: ... Sequencing -- In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome -- Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted Pre-Amplification and Next Generation Y-STR...

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Chromosome Architecture [E-Book] : Methods and Protocols /
Table of Contents: ...New Advances in Chromosome Architecture -- Single Molecule Narrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose Signal Transduction Of Live Yeast Cells -- Single-Molecule Imaging to Characterise the Transport Mechanism of the Nuclear Pore...

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Clostridium difficile [E-Book] : Methods and Protocols /
Table of Contents: ... in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection...

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