Table of Contents: “... Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear...”

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Table of Contents: “...Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors -- Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line -- Establishment of Stable Cell Lines from Primary Human...”

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Table of Contents: “... of 3'UTR of mRNA for Expression Knockdown -- Quantitative Evaluation of Exon Skipping in Immortalized Muscle Cells In Vitro -- Direct Reprogramming of Human DMD Fibroblasts into Myotubes for In Vitro Evaluation of Antisense-m Exon Skipping and Exons 45-55...”

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Table of Contents: “...Culture of Human Monocyte-Derived Macrophages -- A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages -- Growing Murine Bone Marrow-Derived Macrophages -- Immortalization of Murine Bone Marrow...”

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Table of Contents: “... -- Differentiation and Metabolic Interrogation of Human Adipocytes -- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines -- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue -- Genetic Mouse...”

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Table of Contents: “... and Characterization of Exosomes Released by EBV Immortalized Cells -- Functional Analysis of Exosomes Derived from EBV-Infected Cells -- Terminal Repeat Analysis of EBV Genomes -- Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA...”

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Table of Contents: “...The Immortal Senescence -- Senescence Phenotypes Induced by Ras in Primary Cells -- Cellular Model of p21-Induced Senescence -- Detecting Markers of Therapy-Induced Senescence in Cancer Cells -- Genome-Wide miRNA Screening for Genes Bypassing Oncogene...”

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Table of Contents: “...Preface -- Introduction -- Antigravity -- Space travel -- Aliens -- Time travel -- The Nature of Reality -- Invisibility -- Robots -- Transportation -- Immortality -- Mad Scientists -- Epilogue: a New Default Future? -- Index....”

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Table of Contents: “... -- Isolation, Expansion And Immortalization Of Human Adipose-Derived Mesenchymal Stromal Cells From Biopsies And Liposuction Specimens -- Optimization Of Mesenchymal Stem Cells To Increase Their Therapeutic Potential -- Directed Differentiation Of Human Induced...”

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Table of Contents: “...CRISPR/Cas9 mediated Genome-editing of Mouse Small Intestinal Organoids -- Lentivirus-based Stable Gene Delivery into Intestinal Organoids -- Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells -- An Air-Liquid Interface Culture...”

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Table of Contents: “... Construction, Library Screening and Affinity Maturation -- Human B Cell Immortalization for Monoclonal Antibody Production -- Using Next-generation Sequencing for Discovery of High-frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice...”

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Table of Contents: “... -- The Fine-Tuning Conjecture -- Part III Our Future in the Universe -- The Future of Scientific Simulations -- Cosmological Selections -- High Energy Astrobiology -- Cosmological Ethics and Immortality -- Appendix I: A Cosmic Evolutionary Worldview -- Appendix II...”

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Table of Contents: “... and dangers -- Genetic enhancement: humankind healing and redesigning itself -- Human reproductive cloning: sameness, uniqueness, and personal identity -- Age retardation: chasing immortality for better or worse -- The mind: neuroenhancement and neuroethics...”

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Table of Contents: “... Infection -- Gene Targeting in Mice: A Review -- A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells -- Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells....”

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