Table of Contents: “... Vector Construction -- Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9 -- CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants -- CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells -- Cell-Type...”

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Table of Contents: “... Single Base Editing in Rice Plant at More than One Locus -- 4. CRISPR-Cas9 Mediated Genome Editing of the Model Grass Species Brachypodium distachyon -- 5. CRISPR/Cas9-mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora -- 6...”

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Table of Contents: “... that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection -- Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection...”

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Table of Contents: “... PCR (qPCR) -- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) -- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine -- A General Protocol...”

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Table of Contents: “... in Design of Synthetic Peptide Vaccine Against Influenza Virus -- A New Approach to Assess mAb Aggregation -- Generation of Variability-Free Reference Proteomes from Pathogenic Organisms for Epitope-Vaccine Design -- Immunoinformatic Identification of Potential...”

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Table of Contents: “...- and Short- Term Adaptation in Maize and Teosinte -- Neurospora from Natural Populations: Population Genomics Insights Into the Life History of a Model Microbial Eukaryote -- Population Genomics of Fungal Plant Pathogens and the Analyses of Rapidly Evolving Genome...”

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Table of Contents: “...Genetic Association Studies in Host-Pathogens Interaction Analysis.-Bacterial Genotyping Methods: From the Basics to Modern -- Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens -- The Usage of a...”

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Table of Contents: “... small RNAs in Bacteria -- Host-pathogen Transcriptomics by Dual RNA-seq -- Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing -- Assessment of External Guide Sequences (EGS) Efficiency as Inducers...”

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Table of Contents: “... with Peptide Nucleic Acid Probes -- High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture -- Pedigree-defined Haplotypes and their Applications to Genetic Studies -- Haplotyping a Non-Meiotic Diploid Fungal Pathogen...”

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Table of Contents: “... Detection of Protein-Metabolite Interactions in Bacteria -- Identifying Bacterial Immune Evasion Proteins using Phage Display -- Competition for Iron between Host and Pathogen: A Structural Case Study on Helicobacter pylori -- Common Challenges in Studying...”

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Table of Contents: “... Pathogens by Real-Time Polymerase Chain Reaction -- Methods to Study Antagonistic Activities Among Oral Bacteria -- Natural Transformation of Oral Streptococci by Use of Synthetic Pheromones -- Markerless Genome Editing in Competent Streptococci -- Tools...”

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Table of Contents: “... Expression in Escherichia coli -- Colony PCR -- CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water -- Multiplex Detection Of Food-borne Pathogen -- Fast Real-time PCR for the Detection of Crustacean Allergens...”

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Table of Contents: “... and Genotyping of Bacterial Pathogens by Pyrosequencing -- Methods for Genotyping-by-Sequencing.-Describing Sequence Variants Using HGVS Nomenclature....”

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