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Table of Contents: ... -- Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design -- Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA -- Gene targeting in mouse embryonic stem cells via CRISPR/Cas9...

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Table of Contents: ... in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts -- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus -- Studying the Organization of the Golgi by Super-Resolution Microscopy -- Super-Resolution Live Imaging...

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Table of Contents: ... -- Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells -- Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments -- Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags...

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Table of Contents: ... -- Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells -- Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids -- A Three-Dimensional...

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Table of Contents: ... using Fluorescent Probes -- Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis -- Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions -- Histochemical...

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Table of Contents: ... for Enhancing Compound Production -- A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation -- Novel Nanotechnology-Driven Prototypes for AI-Enriched Implanted Prosthetics Following Organ Failure -- Simplified CRISPR...

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Table of Contents: ... Cells -- Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells -- Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation -- Chondrogenic Differentiation of Human Induced Pluripotent Stem...

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Table of Contents: ... Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using...

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Table of Contents: ... Alterations in Alzheimer's Disease -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer's Disease -- Modeling Alzheimer's Disease using Human Brain Organoids -- 3D Biohybrid StarPEG...

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Table of Contents: ...Differentiation of Human Pluripotent Stem cells (hPSCs) into Brown-Like Adipocytes -- Culture and Differentiation of Primary Preadipocytes from Mouse Subcutaneous White Adipose Tissue -- Isolation and Differentiation of Adipocyte Precursors Derived from...

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Table of Contents: ... Locus -- Improved Genome Editing via Oviductal Nucleic Acids Delivery (i-GONAD): Protocol Steps and Additional Notes -- Gene Targeting in Rat Embryonic Stem Cells -- Rat Embryonic Stem Cell Transgenesis -- CRISPR/Cas9-Mediated Genome Editing in Zebrafish...

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Table of Contents: ...Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells -- Extra Eyelid-Derived Muscle Stem Cells -- Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis...

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Table of Contents: ... Macromolecules -- LINE-1 retrotransposition assays in embryonic stem cells -- Detecting somatic transposable element insertions in Drosophila tissues -- Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering -- Epigenetic editing...

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Table of Contents: ... and preclinical testing -- CRISPR manipulations in stem cell lines -- CRISPR-mediated genome engineering in cell lines -- CRISPR off-targeting -- CRISPR manipulations in stem cell lines -- Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV...

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Table of Contents: ... that Affect PARP1 Activity In Vivo -- Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System -- TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice -- Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells...

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Table of Contents: ... Patients -- Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research -- A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture -- A Step-by-Step Methodological Guide for Developing...

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Table of Contents: ... Bilayers to Probe the Mechanosensitivity of Signaling Receptors -- Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing -- Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK...

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Table of Contents: ... fish:acquisition, decryption, interpretation -- Generation of mini-brains from hiPSCs -- Generating Human Pluripotent Stem Cell-Derived AssemBloids™ to Model Interneuron Migration and Immune Cell Interactions -- Multiplexed quantitative proteomics analysis of developing...

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Table of Contents: ... Using Digenome-Seq -- Base Editing of Human Hematopoietic Stem Cells -- A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells -- Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors -- Use of the Representative Base Editing Tool...

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Table of Contents: ... Expression with Duchenne Muscular Dystrophy Exon 45 Skipping in Induced-Pluripotent Stem Cell-Derived Cardiomyocytes -- Quantitative Evaluation of Exon Skipping in Urine-Derived Cells for Duchenne Muscular Dystrophy -- Use of Glycine to Augment Exon Skipping...

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