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Cancer Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Cancer Stem Cells: Current Challenges and Future Perspectives -- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods -- Isolating Cancer Stem Cells from Solid Tumors -- Surface Markers for the Identification of Cancer Stem Cells...

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Tau Protein [E-Book] : Methods and Protocols /
Table of Contents: ... with Noradrenergic, Dopaminergic, and Cholinergic Properties -- Cultivation, Differentiation, and Lentiviral Transduction of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Glutamatergic Neurons for Studying Human Tau -- Optimized Calcium-Phosphate Based Co...

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Epithelial Cell Culture [E-Book] : Methods and Protocols /
Table of Contents: ... Liver Injury -- In Vitro Porcine (Explant) Colon Culture -- Bioencapsulation of Oocytes and Granulosa Cells -- Culturing and Differentiation of Patient-Derived Ectocervical Epithelial Stem Cells using Air-Liquid Interphase and Matrigel Scaffold -- Ovine...

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Mammalian Synthetic Systems [E-Book] /
Table of Contents: ... of mRNA capping level -- In vitro generation of megakaryocytes from engineered mouse embryonic stem cells -- Preparation of chromobodies for the detection of cell surface epitopes....

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Neurobiology [E-Book] : Methods and Protocols /
Table of Contents: ... chimeric embryo and stem-cell based neuruloid model -- Active induction of a multiple sclerosis-like disease in common laboratory mouse strains.-Immunohistochemical analysis of the Drosophila Larval Neuromuscular Junction -- Neural endophenotype assessment...

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Mouse Models of Cancer [E-Book] : Methods and Protocols /
Table of Contents: ...In Vivo Modeling of Leukemia by Murine Stem Cell Transplantation -- NOTCH1-Induced T-Cell Acute Lymphoblastic Leukemia In Vivo Models -- Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine -- Establishment of Mouse Orthotopic Urinary...

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Embryo Models In Vitro [E-Book] : Methods and Protocols /
Table of Contents: ...Generation of Human Blastoids from Naïve Pluripotent Stem Cells -- Human Pre-Gastrulation Embryo Culture in 3D Condition -- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System -- Generation of Human...

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Zebrafish [E-Book] : Methods and Protocols /
Table of Contents: ... of Regenerating Fins -- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein -- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones -- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools...

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Cell Cycle Control [E-Book] : Methods and Protocols /
Table of Contents: ... Organisms -- Whole Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis -- Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division -- Cell Cycle Mapping Using Multiplexed Immunofluorescence -- Investigating...

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Adipose-Derived Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation of Human Adipose-Derived Stem Cells -- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry -- Soft Tissue Reconstruction...

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Maternal Placental Interface [E-Book] : Methods and Protocols /
Table of Contents: ... Decidual Cells from the Fetal Membranes of Term Placentae -- Generation of knockout mouse trophoblast stem cells by CRISPR/Cas -- CRISPR-activation in mouse trophoblast stem cells -- Feto-maternal interface organ-on-chip: a new technology to study ascending...

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Rheumatoid Arthritis [E-Book] : Methods and Protocols /
Table of Contents: ... Docking Calculations -- RNA interference ex vivo -- Lentiviral-mediated systemic RNA interference in vivo -- Lentiviral production platform -- Mesenchymal stem cell engineering -- Screening of Ca2+ influx in lymphocytes -- Single-cell Ca2+ imaging...

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Teratogenicity Testing [E-Book] : Methods and Protocols /
Table of Contents: ... -- The First Steps on AOPs' Concepts, Development, and Applications in Teratology -- Machine Learning to Predict Teratogenicity: Theory and Practice -- Human Pluripotent Stem Cell-Based Assays to Predict Developmental Toxicity -- Developmental Toxicity Using the Rat...

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Germ Cell Development [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation and Purification of Viable PGCs from Mouse Embryos -- Purification and Transfection Methods of Chicken Primordial Germ Cells -- Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells -- Purification by STA-PUT Technique of Male...

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Stem Cells and Lineage Commitment [E-Book] : Methods and Protocols /
Table of Contents: ...Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment -- Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin -- Targeted Gene Silencing by Using...

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Trophoblasts [E-Book] : Methods and Protocols /
Table of Contents: ... to Study Human Placental Development and Function -- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening -- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem...

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Familial Cardiomyopathies [E-Book] : Methods and Protocols /
Table of Contents: ... -- Genomic Engineering of Induced Pluripotent Stem Cell-Derived Cardiomyocytes -- The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses -- Motility Assay to Probe the Calcium Sensitivity of Myosin...

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Tissue-Resident Macrophages [E-Book] : Methods and Protocols /
Table of Contents: ... Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell...

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Brain Organoid Research [E-Book] /
Table of Contents: ... -- Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells -- Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids -- A Three-Dimensional...

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Histochemistry of Single Molecules [E-Book] : Methods and Protocols  /
Table of Contents: ... using Fluorescent Probes -- Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis -- Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions -- Histochemical...

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