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Human Naïve Pluripotent Stem Cells [E-Book] /
Table of Contents: ...Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition -- Induction of Human Naïve Pluripotency Using 5i/L/A Medium -- Induction of Human Naïve Pluripotency Using Chemical...

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Cell Penetrating Peptides [E-Book] : Methods and Protocols  /
Table of Contents: ... with PepFects by organic and inorganic components -- DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent -- Use of PepFect14 and siRNA for targeted gene silencing in human embryonic stem cells -- CRISPR/Cas9 plasmid delivery through...

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Single-Cell Protein Analysis [E-Book] : Methods and Protocols  /
Table of Contents: ... and Surface Protein Analysis Using the Mission Bio Tapestri Platform -- Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform -- Generation...

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Vascular Tissue Engineering [E-Book] : Methods and Protocols /
Table of Contents: ...Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling -- Generating Monocyte Derived Endothelial-Like Cells for Vascular Regeneration -- Methods for Differentiating...

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Vascular Morphogenesis [E-Book] : Methods and Protocols /
Table of Contents: ... of Angiogenesis -- Endothelial Cells: Co-Culture Spheroids -- Microfluidic Device Setting by Co-Culturing Endothelial Cells and Mesenchymal Stem Cells -- Spatial Statistics-Based Image Analysis Methods for the Study of Vascular Morphogenesis -- Studying Angiogenesis...

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The Integrin Interactome [E-Book] : Methods and Protocols /
Table of Contents: ... of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation -- Quantitative Analysis of Integrin Trafficking -- Methods to Study Integrin Functions on Exosomes -- Functional Bioinformatics Analyses of the Matrisome...

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Germline Development in the Zebrafish [E-Book] : Methods and Protocols /
Table of Contents: ... -- Cryopreservation and Transplantation of Spermatogonial Stem Cells -- Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos -- Chemical Genetics: Manipulating the Germline with Small...

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Mitochondrial Regulation [E-Book] : Methods and Protocols /
Table of Contents: ... and Mitochondrial Function -- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs) -- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes -- Update on the Histoenzymatic Methods for Visualization of the Activity...

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Pericytes [E-Book] : Methods and Protocols /
Table of Contents: ... Pericytes and the Regulation of Cerebral Blood Flow -- Derivation of Pericytes from Human Pluripotent Stem Cells -- Assessing the Bone-Forming Potential of Pericytes -- Manipulating Pericyte Function with MicroRNAs -- Isolation of Mesoangioblasts: A Subset...

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Myofibroblasts [E-Book] : Methods and Protocols /
Table of Contents: ... of Renal Fibrosis in Human Pluripotent Stem Cell-Derived Kidney Organoids -- Decellularized Human Lung Scaffolds as Complex Three-Dimensional Tissue Culture Models to Study Functional Behavior of Fibroblasts....

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The Epithelial-to Mesenchymal Transition [E-Book] : Methods and Protocols /
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Cell Reprogramming for Immunotherapy [E-Book] : Methods and Protocols /
Table of Contents: ... Immune Cells by Positron Emission Tomography -- Functional Analysis of Human Hematopoietic Stem Cells in vivo in Humanized Mice -- Monitoring Allogeneic CAR-T Cells using Flow Cytometry -- Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma...

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Cell Tracking [E-Book] : Methods and Protocols  /
Table of Contents: ...Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo -- Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations -- In vivo Epithelial Metabolic Imaging using a Topical Fluorescent Glucose Analog...

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Epidermal Cells [E-Book] : Methods and Protocols /
Table of Contents: ... and Characterization of Extracellular Vesicles from Keratinocyte Cultures -- Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential -- Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis...

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Basophils and Mast Cells [E-Book] : Methods and Protocols /
Table of Contents: ... Mast Cell Phenotype from CD133+ Stem Cells -- Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs) -- Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34...

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Intestinal Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy -- Immune-Mediated Specific Depletion of Intestinal Stem Cells -- Analysis of Aged Dysfunctional Intestinal Stem Cells -- Strategies for Measuring Induction of Fatty Acid...

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Molecular Dermatology [E-Book] : Methods and Protocols  /
Table of Contents: ...: A Model for Alopecia Areata -- Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo -- Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells -- Method...

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Planarian Regeneration [E-Book] : Methods and Protocols /
Table of Contents: ...Planarian Diversity and Phylogeny -- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale -- The Ecology of Freshwater Planarians -- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key...

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Schwann Cells [E-Book] : Methods and Protocols /
Table of Contents: ... Schwann Cells -- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats -- Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells...

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Hypoxia [E-Book] : Methods and Protocols /
Table of Contents: ... -- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens -- Isolation of GBM Stem Cells with Flow Cytometry -- Lentiviral Transduction of Primary Human Glioblastoma Cultures -- Selective Targeting of CD133-expressing Glioblastoma Stem...

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