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Embryo Models In Vitro [E-Book] : Methods and Protocols /
Table of Contents: ...Generation of Human Blastoids from Naïve Pluripotent Stem Cells -- Human Pre-Gastrulation Embryo Culture in 3D Condition -- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System -- Generation of Human...

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Gene, Drug, and Tissue Engineering [E-Book] /
Table of Contents: ... for Enhancing Compound Production -- A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation -- Novel Nanotechnology-Driven Prototypes for AI-Enriched Implanted Prosthetics Following Organ Failure -- Simplified CRISPR...

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Cartilage Tissue Engineering [E-Book] /
Table of Contents: ... Cells -- Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells -- Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation -- Chondrogenic Differentiation of Human Induced Pluripotent Stem...

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Somatic Cell Nuclear Transfer Technology [E-Book] /
Table of Contents: ... following nuclear transfer: from cloned animals to patients with mitochondrial disease -- Stem cells as nuclear donors for mammalian cloning -- Animal Transgenesis and cloning: combined development and future perspectives -- Mouse cloning using outbred oocyte...

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Human Naïve Pluripotent Stem Cells [E-Book] /
Table of Contents: ...Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition -- Induction of Human Naïve Pluripotency Using 5i/L/A Medium -- Induction of Human Naïve Pluripotency Using Chemical...

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Bioreactors in Stem Cell Biology [E-Book] : Methods and Protocols  /
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Induced Pluripotent Stem Cells and Human Disease [E-Book] : Methods and Protocols /
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Vascular Tissue Engineering [E-Book] : Methods and Protocols /
Table of Contents: ...Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling -- Generating Monocyte Derived Endothelial-Like Cells for Vascular Regeneration -- Methods for Differentiating...

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Cardiac Tissue Engineering : Methods and Protocols [E-Book] /
Table of Contents: ...CRISPR Library Screening in Cultured Cardiomyocytes -- Protein and mRNA Quantification in Small Samples of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates -- Self-Assembled Heterotypic Cardiac Spheroids from Human...

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Whole-Body Regeneration [E-Book] : Methods and Protocols /
Table of Contents: ... using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression...

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Next Generation Culture Platforms for Reliable In Vitro Models [E-Book] : Methods and Protocols /
Table of Contents: ..., Large, Uniform Spheroids and Organoids -- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation -- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic...

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Myofibroblasts [E-Book] : Methods and Protocols /
Table of Contents: ... of Renal Fibrosis in Human Pluripotent Stem Cell-Derived Kidney Organoids -- Decellularized Human Lung Scaffolds as Complex Three-Dimensional Tissue Culture Models to Study Functional Behavior of Fibroblasts....

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Skeletal Development and Repair [E-Book] : Methods and Protocols /
Table of Contents: ... and Stem Cell Quiescence in Skeletal Tissue Sections -- Whole-mount In Situ Hybridization in Murine Tissues -- Bone Marrow Stromal Cell Assays - In Vitro and In Vivo -- Isolation and Culture of Periosteum Derived Progenitor Cells from Mice -- Isolation...

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Wound Regeneration [E-Book] : Methods and Protocols  /
Table of Contents: ... of Dental Pulp-Derived Stem Cell Product for Healing -- Musculoskeletal Tissue Engineering Using Fibrous Biomaterials -- Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy -- Contusion Rodent...

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Cardiac Regeneration [E-Book] : Methods and Protocols  /
Table of Contents: ... Myocardial Infarction and Regeneration -- Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes -- Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes -- Differentiation of Human Induced Pluripotent Stem...

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Current Applications of Pharmaceutical Biotechnology [E-Book] /
Table of Contents: ... -- Advances in Vaccines -- Human Pluripotent Stem Cells: Applications and Challenges for Regenerative Medicine and Disease Modeling -- Addressing the Manufacturing Challenges of Cell-Based Therapies -- Bioprinting Technologies in Tissue Engineering -- Gene Therapy....

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Corneal Regeneration [E-Book] : Methods and Protocols  /
Table of Contents: ...Isolation and Culture of Corneal Stromal Stem Cells -- In vitro Expansion of Corneal Endothelial Cells for Transplantation -- Primary Culture of Cornea-Limbal Epithelial Cells In Vitro -- Optimization of Human Limbal Stem Cell Culture by Replating a...

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Stem Cells and Tissue Repair [E-Book] : Methods and Protocols /
Table of Contents: ...Culturing and Manipulating Mouse Embryonic Stem Cells -- Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter -- Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair -- In Vitro Direct...

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Advances in Biomaterials for Biomedical Applications [E-Book] /
Table of Contents: ...State of the Art and Future Directions of Macroporous Cryogels in Tissue Engineering -- 3-D Printed Biomaterials -- Biomaterials Adapted for Stem Cells Dedicated to Cartilage Engineering -- Polyelectrolyte complexes (PECs) for biomedical applications...

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Stem cell biology and regenerative medicine [E-Book] /