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Cancer Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Cancer Stem Cells: Current Challenges and Future Perspectives -- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods -- Isolating Cancer Stem Cells from Solid Tumors -- Surface Markers for the Identification of Cancer Stem Cells...

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Adipose-Derived Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation of Human Adipose-Derived Stem Cells -- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry -- Soft Tissue Reconstruction...

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Stem Cells and Lineage Commitment [E-Book] : Methods and Protocols /
Table of Contents: ...Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment -- Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin -- Targeted Gene Silencing by Using...

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Hematopoietic Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...The Factory of Blood Production: Hematopoietic Stem Cells -- Detection of DNA Damage in Hematopoietic Stem Cells -- Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation -- CRISPR Gene Editing...

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Germline Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Analysis of the C. elegans Germline Stem Cell Pool -- Analysis of C. elegans Germline Small RNA Pathways -- Lineage Tracing and Single Cell RNA-seq in C. elegans to Analyze Transgenerational Epigenetic Phenotypes Inherited from Germ Cells -- Methods...

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Thermogenic Fat [E-Book] : Methods and Protocols  /
Table of Contents: ...Differentiation of Human Pluripotent Stem cells (hPSCs) into Brown-Like Adipocytes -- Culture and Differentiation of Primary Preadipocytes from Mouse Subcutaneous White Adipose Tissue -- Isolation and Differentiation of Adipocyte Precursors Derived from...

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Skeletal Muscle Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells -- Extra Eyelid-Derived Muscle Stem Cells -- Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis...

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Somatic Cell Nuclear Transfer Technology [E-Book] /
Table of Contents: ... following nuclear transfer: from cloned animals to patients with mitochondrial disease -- Stem cells as nuclear donors for mammalian cloning -- Animal Transgenesis and cloning: combined development and future perspectives -- Mouse cloning using outbred oocyte...

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Spermatogonial Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Introduction: The Why's and How's for Studying Spermatogenesis and Spermatogonial Stem Cells -- Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia -- Single-Molecule Fluorescence...

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Stem Cell-Based Neural Model Systems for Brain Disorders [E-Book] /
Table of Contents: ...Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture -- Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells -- Generation and Co-Culture of Cortical Glutamatergic...

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Neural Progenitor Cells [E-Book] : Methods and Protocols /
Table of Contents: ...-Floating Neurospheres -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord -- Culturing and Expansion of "Clinical Grade" Neural Stem Cells from the Fetal Human Central Nervous System -- Microglia Isolation from Neural Stem Cell-Enriched...

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Human Naïve Pluripotent Stem Cells [E-Book] /
Table of Contents: ...Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition -- Induction of Human Naïve Pluripotency Using 5i/L/A Medium -- Induction of Human Naïve Pluripotency Using Chemical...

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Mammary Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ... starting with the embryo -- Computational methods to identify cell fate determinants, identity transcription factors and niche induced signaling pathways for stem cell research -- Differential proteomic analysis of complex mixtures by label free nLC MS/MS...

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Bioreactors in Stem Cell Biology [E-Book] : Methods and Protocols  /
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Epiblast Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Establishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification...

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Stem Cell Assays [E-Book] : Methods and Protocols  /
Table of Contents: ...Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells -- Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes -- Generation of Human iPSC from Small Volume Peripheral...

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Non-Alcoholic Steatohepatitis [E-Book] : Methods and Protocols  /
Table of Contents: ... of Human Induced Pluripotent Stem Cells-Derived Liver Organoids -- Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver -- Analysis of Liver Responses to Non-Alcoholic Steatohepatitis by mRNA-Sequencing -- Single-Cell RNA-Sequencing...

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Whole-Body Regeneration [E-Book] : Methods and Protocols /
Table of Contents: ... using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression...

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Induced Pluripotent Stem (iPS) Cells [E-Book] : Methods and Protocols  /
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Induced Pluripotent Stem Cells and Human Disease [E-Book] : Methods and Protocols /