Protein Acetylation [E-Book] : Methods and Protocols / edited by Sandra B. Hake, Christian J. Janzen.
Hake, Sandra B.
Janzen, Christian J.
Totowa, NJ : Humana Press, 2013
XI, 266 p. 54 illus., 18 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 981
Full Text
Table of Contents:
  • Validation of Protein Acetylation by Mass Spectrometry
  • Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery
  • Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites
  • Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations
  • Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin
  • Identification and Analysis of O-acetylated Sialoglycoproteins
  • HPLC-based Quantification of in vitro N-terminal Acetylation
  • Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography
  • In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation
  • Computational Prediction of Lysine Acetylation Proteome-wide
  • Generation and Characterization of Pan-specific Anti-acetyllysine Antibody
  • Using Functional Proteome Microarrays to Study Protein Lysine Acetylation
  • Quantitation of Nucleosome Acetylation and other Histone Post-Translational Modifications Using Microscale NU-ELISA
  • Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core
  • Production of Amino-terminally Acetylated Recombinant Proteins in E. coli
  • Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics
  • Non-radioactive in-vitro Assays for Histone Deacetylases
  • The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes
  • Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators.