Yeast Protocols [E-Book] / edited by Wei Xiao.
Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and...
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Full text |
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Personal Name(s): | Xiao, Wei. editor |
Edition: |
3rd ed. 2014. |
Imprint: |
New York, NY :
Humana Press,
2014
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Physical Description: |
X, 313 p. 57 illus., 27 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493907991 |
DOI: |
10.1007/978-1-4939-0799-1 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
1163 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- Basic Investigations in Saccharomyces cerevisiae
- Isolation of Yeast Nucleic Acids
- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry
- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry
- Yeast Two Hybrid Liquid Screening
- Targeted Mutagenesis of a Specific Gene in Yeast
- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae
- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy
- Assays for Autophagy II: Mitochondrial Autophagy
- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction
- Yeast Survival and Growth Assays
- Spontaneous Mutagenesis Assay
- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene
- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe
- Replicative and Chronological Life Span Assays
- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
- Ligand Engineering Using Yeast Surface Display
- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.