Opioid Receptors [E-Book] : Methods and Protocols / edited by Santi M. Spampinato.
Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and traffic...
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Full text |
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Personal Name(s): | Spampinato, Santi M. editor |
Imprint: |
New York, NY :
Humana Press,
2015
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Physical Description: |
XV, 326 p. 56 illus., 26 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493917082 |
DOI: |
10.1007/978-1-4939-1708-2 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
1230 |
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- Overview of Genetic Analysis of Human Opioid Receptors
- Computational Structural Biology of Opioid Receptors
- Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription
- Renilla Luciferase Reporter Assay to Study 3’UTR-Driven Post-Transcriptional Regulations of OPRM1
- High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas
- Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy
- In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors
- Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins
- Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR)
- Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-µC, an Affinity-Purified Polyclonal Anti-MOPR Antibody
- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
- [35S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality
- Fluorescence-Based, High-Throughput Assays for µ-Opioid Receptor Activation Using Membrane Potential-Sensitive Dye
- Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors
- Electrophysiological Patch Clamp Assay to Monitor the Action of Opioid Receptors
- Skin-Nerve Preparation to Assay the Function of Opioid Receptors in Peripheral Endings of Sensory Neurons
- Mechanical Nociception Measurement in Mice and Rats with Automated Von Frey Equipment
- Detecting Zinc Release Induced by Mu-Opioid Receptor Agonists in Brain Slices
- Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye
- Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment
- Measurement of Macrophage Toll Like Receptor 4 Expression After Morphine Treatment
- The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts
- Role of Opioid Receptors in the Reinstatement of Opioid-Seeking Behavior: An Overview
- Analysis of Opioid-Seeking Reinstatement in the Rat
- Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors
- Evaluation of Social and Non-Social Behaviors Mediated by Opioids in Mouse Pups.