The Genetic Manipulation of Staphylococci [E-Book] : Methods and Protocols / edited by Jeffrey L. Bose.
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended chang...
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Full text |
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Personal Name(s): | Bose, Jeffrey L. editor |
Imprint: |
New York, NY :
Humana Press,
2016
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Physical Description: |
X, 186 p. 23 illus., 7 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493931583 |
DOI: |
10.1007/978-1-4939-3158-3 |
Series Title: |
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Methods in molecular biology ;
1373 |
Subject (LOC): |
- Understanding Staphylococcal Nomenclature
- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus
- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci
- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning
- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
- Method for Preparation and Electroporation of S. aureus and S. epidermidis
- Rapid Isolation of DNA from Staphylococcus
- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method
- Bacteriophage Transduction in Staphylococcus aureus
- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
- Conjugative Transfer in Staphylococcus aureus
- Allelic Exchange
- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917
- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis
- Chemical and UV Mutagenesis
- Pulsed Field Gel Electrophoresis
- RNA-Sequencing of Staphylococcus aureus Messenger RNA
- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression
- Electrophoretic Mobility Shift Assays
- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.