Nucleic Acid Crystallography [E-Book] : Methods and Protocols / edited by Eric Ennifar.
Ennifar, Eric. (editor)
1st ed. 2016.
New York, NY : Humana Press, 2016
XI, 356 p. 116 illus., 98 illus. in color. online resource.
Methods in molecular biology ; 1320
Full Text
Table of Contents:
  • Perspectives and Pitfalls in Nucleic Acids Crystallography
  • Preparation of Short 5’-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies
  • Preparation and Crystallization of Riboswitches
  • In Vitro/In Vivo Production of tRNA for X-Ray Studies
  • Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA
  • Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs
  • Fab Chaperone-Assisted RNA Crystallography (Fab CARC)
  • Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes
  • Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes
  • Crystallographic Data and Model Quality
  • Advanced Crystallographic Data Collection Protocols for Experimental Phasing
  • Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases
  • Practical Radiation Damage Induced Phasing
  • Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data
  • Using Molecular Replacement Phasing to Study the Structure and Function of RNA
  • Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?
  • RNA Structure Refinement Using the ERRASER-Phenix Pipeline
  • Neutron Nucleic Acid Crystallography
  • Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins
  • Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers
  • Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor
  • Anions in Nucleic Acid Crystallography.