Cyclin-Dependent Kinase (CDK) Inhibitors [E-Book] : Methods and Protocols / edited by Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá.
Orzáez, Mar. (editor)
Pérez-Payá, Enrique. (editor) / Sancho Medina, Mónica. (editor)
1st ed. 2016.
New York, NY : Humana Press, 2016
X, 227 p. 42 illus., 22 illus. in color. online resource.
englisch
9781493929269
10.1007/978-1-4939-2926-9
Methods in molecular biology ; 1336
Full Text
Table of Contents:
  • Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity
  • Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
  • Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9
  • Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination
  • Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors
  •  Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors
  • Identification of Cyclin A Binders with a Fluorescent Peptide Sensor
  • Cell Synchronization Techniques to Study the Action of CDK Inhibitors
  • Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis
  • Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models
  • Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair
  • Drug Delivery Strategies of Chemical CDK Inhibitors
  • Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs
  • Evaluating Chemical CDK Inhibitors as Cell Death Inducers
  • Models for the Study of the Crosstalk between Inflammation and Cell Cycle
  • Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors.