The Mitotic Spindle [E-Book] : Methods and Protocols / edited by Paul Chang, Ryoma Ohi.
This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that...
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Full text |
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Personal Name(s): | Chang, Paul, editor |
Ohi, Ryoma, editor | |
Imprint: |
New York, NY :
Humana Press,
2016
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Physical Description: |
XIII, 424 p. 79 illus., 52 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493935420 |
DOI: |
10.1007/978-1-4939-3542-0 |
Series Title: |
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Methods in molecular biology ;
1413 |
Subject (LOC): |
- Using Fluorescence Microscopy to Study Mitosis
- Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation
- Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts
- Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
- Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles
- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy
- Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size
- In Vitro Kinetochore Assembly
- Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes
- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells
- Studying Kinetochores In Vivo Using FLIM-FRET
- Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies
- A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion
- Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
- Analyzing Spindle Positioning Dynamics in Cultured Cells
- Quantification of Mitotic Chromosome Alignment
- Imaging Mitosis in the Moss Physcomitrella Patens
- Small Molecule Approach to Study the Function of Mitotic Kinesins
- Identification and Characterization of Mitotic Spindle-Localized Transcripts
- Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization
- Studying Kinetochore Kinases
- Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics
- Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer
- Generation and Purification of Tetraploid Cells
- Anti-Microtubule Drugs.