Vaccine Adjuvants [E-Book] : Methods and Protocols / edited by Christopher B. Fox.
Fox, Christopher B., (editor)
New York, NY : Humana Press, 2017
XII, 372 p. 75 illus., 33 illus. in color. online resource.
Methods in molecular biology ; 1494
Full Text
Table of Contents:
  • Overview of Vaccine Adjuvants: Introduction, History, and Current Status
  • Development of the CpG Adjuvant 1018: A Case Study
  • Syntheses of Human TLR8-Specific Small-Molecule Agonists
  • Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21
  • QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes
  • Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography
  • Biosynthetic Approaches to Squalene Production: The Case of Yeast
  • In Silico Adjuvant Design and Validation
  • Manufacturing Methods for Liposome Adjuvants
  • Synthesis of Lymph Node-Targeting Adjuvants
  • Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination
  • Manufacture of Oil-in-Water Emulsion Adjuvants
  • Methods to Prepare Aluminum Salt-Adjuvanted Vaccines
  • Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines
  • Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product
  • Stressed Stability Techniques for Adjuvant Formulations
  • Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA)
  • Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling
  • Determination of Protein Content in Alhydrogelâ-Based Vaccines by O-Phthalaldehyde Assay
  • Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference
  • Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques
  • Screening Vaccine Formulations in Fresh Human Whole Blood
  • Analysis of the Innate Response to Adjuvants: Characterization of the Draining Lymph Node by Fluorescence-Activated Cell Sorting
  • Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants
  • Eliciting Epitope-Specific CD8 T Cell Response by Immunization with Microbial Protein Antigens Formulated with -Galactosylceramide: Theory, Practice, and Protocols
  • Molecular Methods and Bioinformatic Tools for Adjuvant Characterization by High-Throughput Sequencing.