Mitochondria [E-Book] : Practical Protocols / edited by Dejana Mokranjac, Fabiana Perocchi.
Mokranjac, Dejana, (editor)
Perocchi, Fabiana, (editor)
2nd ed. 2017.
New York, NY : Humana Press, 2017
XI, 409 p. 77 illus., 46 illus. in color. online resource.
englisch
9781493968244
10.1007/978-1-4939-6824-4
Methods in molecular biology ; 1567
Full Text
Table of Contents:
  • A Guide to Computational Methods for Predicting Mitochondrial Localization
  • Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
  • Isolation of Mitochondria From Saccharomyces Cerevisiae
  • Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
  • Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
  • Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and Total Protein Approach
  • Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
  • Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
  • Chemical Crosslinking in Intact Mitochondria
  • Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
  • Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
  • Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
  • Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer
  • Application of FRET-Based Biosensor Ateam for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
  • A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
  • New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans
  • Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells
  • Analysis of Yeast Mitochondria by Electron Microscopy
  • Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography
  • Assays for Mitophagy in Yeast
  • Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans
  • Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
  • Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq
  • Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines. .