Analysis of Sterols [E-Book] / by L. John Goad, Toshihiro Akihisa
Knowledge is of two kinds. We know a subject ourselves, or we know where we can find information on it Samuel Johnson, 18 April, 1775* Sterols are among the most studied groups of natural products with interest commencing in the 19th century and running to the present. Investigations have embraced t...
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Full text |
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Personal Name(s): | Goad, L. John, author |
Akihisa, Toshihiro, author | |
Imprint: |
Dordrecht :
Springer Netherlands,
1997
|
Physical Description: |
XVIII, 438 p. online resource. |
Note: |
englisch |
ISBN: |
9789400914476 |
DOI: |
10.1007/978-94-009-1447-6 |
Subject (LOC): |
- 1 Nomenclature and biosynthesis of sterols and related compounds
- 1.1 Introduction
- 1.2 IUPAC-IUB recommended nomenclature for steroids and triterpenoids
- 1.3 The biosynthesis of sterols and triterpenes
- 2 Extraction of sterols from tissues
- 2.1 Introduction
- 2.2 Extraction methods
- 2.3 Separation of sterols from extracted lipids
- 2.4 Examples of the extraction of sterols
- References
- 3 Initial separation methods
- 3.1 Introduction
- 3.2 Digitonin precipitation of sterols
- 3.3 Separation of free sterols and steryl conjugates from a total lipid by adsorption column chromatography
- 3.4 Thin-layer chromatography
- 3.5 The use of mini-columns for the preliminary separation of sterol fractions
- 3.6 Hydrolysis of steryl conjugates
- 3.7 Silver nitrate or argentation chromatography
- 3.8 Autoxidation and isomerization of sterols
- References
- 4 High-performance liquid chromatography of sterols
- 4.1 Introduction
- 4.2 Normal- and reversed-phase HPLC
- 4.3 HPLC detectors
- 4.4 HPLC of sterol derivatives
- 4.5 Influence of column temperature on sterol separation by HPLC
- 4.6 Retention data of sterols on HPLC
- 4.7 Sterol separation factors on HPLC
- 4.8 Differentiation of C-24 epimers of 24-alkylsterols on reversed-phase HPLC
- References
- 5 Gas-liquid chromatography of sterols
- 5.1 Introduction
- 5.2 Packed columns and the stationary phase
- 5.3 Capillary columns
- 5.4 Sample injection and detection
- 5.5 Derivatization of sterols
- 5.6 Preparative GC
- 5.7 Retention time data
- 5.8 Separation factors
- 5.9 GC of the C-24 epimers of 24-alkylsterols
- 5.10 GC of steryl conjugates
- References
- 6 Infrared and ultraviolet spectroscopy of sterols
- 6.1 Introduction
- 6.2 Infrared spectroscopy
- 6.3 Ultraviolet spectroscopy
- References
- 7 Mass spectrometry of sterols
- 7.1 Introduction
- 7.2 Sample ionization
- 7.3 Derivatives used for mass spectrometry of sterols
- 7.4 Mass-spectral fragmentations of the sterol rings
- 7.5 Mass-spectral fragmentations of the sterol side chain
- 7.6 Mass spectra of tetracyclic and pentacyclic triterpene alcohols
- 7.7 Mass spectrometry of steryl conjugates
- References
- 8 1H NMR spectroscopy of sterols
- 8.1 Introduction
- 8.2 Methyl signals
- 8.3 Methine and olefinic protons
- 8.4 Quantitative estimation of C-24 epimeric 24-alkylsterol mixtures
- 8.5 Aromatic solvent induced shifts (ASIS)
- 8.6 Lanthanide-induced shifts (LIS)
- References
- 9 13C NMR spectroscopy of sterols
- 9.1 Introduction
- 9.2 Methods for signal assignments
- 9.3 13C Chemical shift data of sterols and triterpene alcohols
- References
- 10 One-dimensional and two-dimensional NMR spectroscopy of sterols
- 10.1 Introduction
- 10.2 ID NMR spectroscopy
- 10.3 2D NMR spectroscopy
- 10.4 Connectivity diagrams for use with 2D NMR data
- 10.5 Generalized 1D and 2D strategies for sterols and related compounds
- 10.6 Complete assignments of 1H NMR spectra of sterols
- References
- 11 X-ray crystallography of sterols
- References
- 12 Sources of sterols
- 12.1 Introduction
- 12.2 4-Desmethylsterols
- 12.3 Large-scale purification of 4-desmethylsterols
- 12.4 Sterols with methyl groups at C-4 and/or C-14
- 12.5 Tetracyclic triterpene alcohols
- 12.6 Pentacyclic triterpene alcohols and ketones
- 12.7 The use of inhibitors of sterol biosynthesis to produce specific sterols
- 12.8 Sources of sterols and tetra- and pentacyclic triterpenes
- References
- Appendix 1 The trivial names of sterols
- Appendix 2 Structures of pentacyclic triterpenes
- Appendix 3 Physical data of selected sterols and triterpenes
- Appendix 4 The quantification of sterols.