Bacterial Protein Secretion Systems [E-Book] : Methods and Protocols / edited by Laure Journet, Eric Cascales.
This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complex...
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Personal Name(s): | Cascales, Eric, editor |
Journet, Laure, editor | |
Imprint: |
New York, NY :
Humana Press,
2017
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Physical Description: |
XVI, 528 p. 96 illus., 70 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493970339 |
DOI: |
10.1007/978-1-4939-7033-9 |
Series Title: |
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Methods in molecular biology ;
1615 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder
- Protein Sorting Prediction
- Cell Fractionation
- Defining Lipoprotein Localisation by Fluorescence Microscopy
- Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate
- Defining Membrane Protein Localization by Isopycnic Density Gradients
- Components Sub-cellular Localisation: Cell Surface Exposure
- Probing Inner Membrane Protein Topology by Proteolysis
- Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM)
- Defining Membrane Protein Topology using pho-lac Reporter Fusions
- In vivo and in vitro Protein-peptidoglycan Interactions
- Measure of Peptidoglycan Hydrolase Activity
- Protein-protein Interaction: Bacterial Two-Hybrid
- Protein-protein Interactions: Yeast two-hybrid
- Protein-protein Interactions: Cytology Two-hybrid
- Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes
- Protein-protein Interactions: Co-immunoprecipitation
- Protein-protein Interaction: Tandem Affinity Purification in Bacteria
- Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes
- Protein-protein Interactions: Pull-down Assays
- Protein-protein interactions: Surface Plasmon Resonance
- Assessing Energy-dependent Protein Conformational Changes in the TonB System
- Defining Assembly Pathways by Fluorescence Microscopy
- Large Complexes: Cloning Strategy, Production, and Purification
- Shearing and Enrichment of Extracellular Type IV Pili
- Blue Native PAGE Analysis of Bacterial Secretion Complexes
- In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography
- Structural Analysis of Protein Complexes by Cryo Electron Microscopy
- Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy
- Identification of Effectors: Precipitation of Supernatant Material
- Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System
- Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot
- Effector Translocation: Cya Reporter Assay
- Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System
- Effector Translocation Assay: Differential Solubilization
- Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture. .