mRNA Decay [E-Book] : Methods and Protocols / edited by Shireen R. Lamandé.
This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein t...
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Full text |
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Personal Name(s): | Lamandé, Shireen R., editor |
Imprint: |
New York, NY :
Humana Press,
2018
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Physical Description: |
XI, 227 p. 34 illus., 24 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493975402 |
DOI: |
10.1007/978-1-4939-7540-2 |
Series Title: |
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Methods in molecular biology ;
1720 |
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- 5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives
- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)
- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay
- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH
- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins
- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs
- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos
- Integration of ENCODE RNAseq and eCLIP Data Sets
- Identifying miRNA Targets Using AgoRIP-Seq.-Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets
- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry
- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay
- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq)
- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.-Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.