Ancient DNA [E-Book] : Methods and Protocols / edited by Beth Shapiro, Axel Barlow, Peter D. Heintzman, Michael Hofreiter, Johanna L. A. Paijmans, André E. R. Soares.
This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. Th...
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Personal Name(s): | Barlow, Axel, editor |
Heintzman, Peter D., editor / Hofreiter, Michael, editor / Paijmans, Johanna L. A., editor / Shapiro, Beth, editor / Soares, André E. R., editor | |
Edition: |
2nd ed. 2019. |
Imprint: |
New York, NY :
Humana Press,
2019
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Physical Description: |
X, 216 pages 15 illustrations, 5 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493991761 |
DOI: |
10.1007/978-1-4939-9176-1 |
Series Title: |
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Methods in Molecular Biology ;
1963 |
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Classification: |
- Setting Up an Ancient DNA Laboratory
- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate
- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step
- Extraction of Highly Degraded DNA from Ancient Bones and Teeth
- Sampling and Extraction of Ancient DNA from Sediments
- Extraction of Ancient DNA from Plant Remains
- DNA Extraction from Keratin and Chitin
- Double-Stranded Library Preparation for Ancient and Other Degraded Samples
- A Method for Single-Stranded Ancient DNA Library Preparation
- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms
- Whole-Genome Capture of Ancient DNA Using Homemade Baits
- Generating RNA Baits for Capture-Based Enrichment
- Hybridization Capture of Ancient DNA Using RNA Baits
- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA
- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis
- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA
- Authentication and Assessment of Contamination in Ancient DNA
- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence.