The ESCRT Complexes [E-Book] : Methods and Protocols / edited by Emmanuel Culetto, Renaud Legouis.
This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalia...
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Full text |
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Personal Name(s): | Culetto, Emmanuel, editor |
Legouis, Renaud, editor | |
Edition: |
1st edition 2019. |
Imprint: |
New York, NY :
Humana Press,
2019
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Physical Description: |
XIV, 322 pages 72 illustrations, 43 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493994922 |
DOI: |
10.1007/978-1-4939-9492-2 |
Series Title: |
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Methods in Molecular Biology ;
1998 |
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Subject (LOC): | |
Classification: |
- Analysis of the Archaeal ESCRT Apparatus
- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster
- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc
- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy
- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells
- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking
- Genetic and Biochemical Analyses of Yeast ESCRT
- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons
- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT
- Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins
- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro
- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production
- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast
- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis
- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana
- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay
- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.