Chimeric Antigen Receptor T Cells [E-Book] : Development and Production / edited by Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Virgínia Picanço-Castro.
Malmegrim, Kelen Cristina Ribeiro, (editor)
Picanço-Castro, Virgínia, (editor) / Swiech, Kamilla, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 274 pages 29 illustrations, 25 illustrations in color (online resource)
Methods in Molecular Biology ; 2086
Full Text
Table of Contents:
  • CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers
  • Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries
  • Phase I/II Manufacture of Lentiviral Vectors under GMP in an Academic Setting
  • Production of Lentivirus for the Establishment of CAR-T Cells
  • Optimized Production of Lentiviral Vectors for CAR-T Cell
  • Lentiviral Vectors Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells
  • In vitro Transcribed (IVT)-mRNA CAR Therapy Development
  • Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors
  • Generation of CAR+ T Lymphocytes using the Sleeping Beauty Transposon System
  • Platforms for Clinical-grade Car-T Cell Expansion
  • CAR-T Cell Expansion in a Xuri Cell Expansion System W25
  • Methods and Process Optimization for Large Scale CAR T Expansion Using the G-Rex Cell Culture Platform
  • Replication Competent Lentivirus Analysis of Vector Transduced T Cell Products used in Cancer Immunotherapy Clinical Trials
  • Immunophenotypic Analysis of CAR-T Cells
  • Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies
  • Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays
  • Analysis of CAR-mediated Tonic Signaling
  • Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model
  • Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors.