Fundamental Approaches to Screen Abnormalities in Drosophila [E-Book] / edited by Monalisa Mishra.
This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, bioch...
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Personal Name(s): | Mishra, Monalisa, editor |
Edition: |
1st edition 2020. |
Imprint: |
New York, NY :
Springer,
2020
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Physical Description: |
VII, 265 pages 116 illustrations, 111 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493997565 |
DOI: |
10.1007/978-1-4939-9756-5 |
Series Title: |
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Springer Protocols Handbooks
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- Chapter 1. Preparation of normal food and modified food for Drosophila
- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly
- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol)
- Chapter 4. Detection of fat within the Drosophila body
- Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells
- Chapter 6. Methods to check the metabolic activity of fly
- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy
- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy
- Chapter 9. A comet assay to detect severity of DNA damage
- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain
- Chapter 11. Analysis of channel proteins
- Chapter 12. Various behavioral assays in larva and adult Drosophila
- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR
- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM)
- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM)
- Chapter 16. Flow cytometric analysis of Drosophila cells.