Axon Degeneration [E-Book] : Methods and Protocols / edited by Elisabetta Babetto.
Babetto, Elisabetta, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 340 pages 80 illustrations, 58 illustrations in color (online resource)
Methods in Molecular Biology ; 2143
Full Text
Table of Contents:
  • Axon Degeneration: Which Method to Choose?
  • Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures
  • Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration
  • Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro
  • Viral Transduction of DRG Neurons
  • Planning and Analysis of Axon Degeneration Screening Experiments
  • A Microfluidic Culture Platform to Assess Axon Degeneration
  • A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration
  • Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination
  • Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage
  • Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function
  • Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction
  • Ex Vivo Studies of Optic Nerve Axon Electrophysiology
  • Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations
  • Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis
  • Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury
  • Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues
  • Transmission Electron Microscopy and Morphometry of the CNS White Matter
  • In Vivo Calcium Imaging During Axon Degeneration in Zebrafish
  • In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves
  • In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons
  • A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster
  • Models of Axon Degeneration in Drosophila Larvae
  • In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster.