TET Proteins and DNA Demethylation [E-Book] : Methods and Protocols / edited by Ozren Bogdanovic, Michiel Vermeulen.
This volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass...
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Full text |
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Personal Name(s): | Bogdanovic, Ozren, editor |
Vermeulen, Michiel, editor | |
Edition: |
1st edition 2021. |
Imprint: |
New York, NY :
Humana Press,
2021
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Physical Description: |
X, 320 pages 57 illustrations, 46 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071612941 |
DOI: |
10.1007/978-1-0716-1294-1 |
Series Title: |
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Methods in Molecular Biology ;
2272 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine
- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine
- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS)
- ELISA-Based Quantitation of Global 5hmC Levels
- Avidin-Biotin ELISA-Based Detection of 5hmC
- Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS
- Distinguishing Active Versus Passive DNA Demethylation using Illumina MethylationEPIC BeadChip Microarrays
- Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions
- TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling
- Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9
- High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation
- Identifying Protein-(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics
- Purification of TET Proteins
- Uncovering Sequence Specific Transcription Factors Interacting with TET2
- ChIP-Sequencing of TET Proteins
- Harnessing Alternative Substrates to Probe TET Family Enzymes
- Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts.