G Protein-Coupled Receptor Screening Assays [E-Book] : Methods and Protocols / edited by Sofia Aires M. Martins, Duarte Miguel F. Prazeres.
This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer...
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Full text |
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Personal Name(s): | Martins, Sofia Aires M., editor |
Prazeres, Duarte Miguel F., editor | |
Edition: |
2nd edition 2021. |
Imprint: |
New York, NY :
Humana Press,
2021
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Physical Description: |
XVI, 327 pages 78 illustrations, 69 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071612217 |
DOI: |
10.1007/978-1-0716-1221-7 |
Series Title: |
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Methods in Molecular Biology ;
2268 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy
- Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing
- Construction of Recombinant Cell Lines for GPCR Expression
- Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor
- Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast
- Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface
- Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples
- Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses
- Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology
- Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction
- Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity
- cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation
- FLIPR Calcium Mobilization Assays in GPCR Drug Discovery
- Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity
- Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins
- NanoLuc-Based Methods to Measure -Arrestin2 Recruitment to G Protein-Coupled Receptors
- Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias
- Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber
- Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays
- Homology Modeling Using GPCRM Web Service.