Analytical Methodologies for Biofilm Research [E-Book] / edited by Moupriya Nag, Dibyajit Lahiri.
The book provides the readers of various discipline an easy understanding of the latest biophysical techniques pertaining to microbiology. Biofilm associated chronic infection is a major health problem and a serious concern to doctors, scientists and other health workers as it develops antibiotic an...
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Personal Name(s): | Lahiri, Dibyajit, editor |
Nag, Moupriya, editor | |
Edition: |
1st edition 2021. |
Imprint: |
New York, NY :
Springer,
2021
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Physical Description: |
VII, 343 pages 56 illustrations, 32 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071613788 |
DOI: |
10.1007/978-1-0716-1378-8 |
Series Title: |
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Springer Protocols Handbooks
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- Section I: Introduction to Biofilms
- Chapter 1: Biofilm formation and pathogenesis
- Chapter 2: Sample preparation, basic equipments and common laboratory assay protocols for Bacterial Communities and Biofilms
- Section II: Separation methods and Spectroscopic techniques in Biofilm research
- Chapter 3: Analysing microbial biofilm formation at a molecular level: Role of Fourier-Transform Infrared and Raman spectroscopy
- Chapter 4: In vitro metabolite profiling of microbial biofilm: Role of Gas Chromatography and High Performance Liquid Chromatograpy
- Section III: Electroanalytical techniques for evaluation of biofilm research
- Chapter 5: Microbial electrochemical cells and introduction to Electron transport in microbial biofilm
- Chapter 6: Contribution of Cyclic voltammetry and electrochemical impedance spectroscopy in deciphering the electron transport system in biofilm
- Section IV: Calorimetric techniques in biofilm research
- Chapter 7: Measuring metabolism and bio-energetic profiles of biofilm: Isothermal calorimetry, Differential Scanning Calorimetry and future of Chip Calorimetry
- Section V: In Situ Surface Analysis and Imaging techniques in Biofilm research
- Chapter 8: Analysis of biofilm matrix by Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) during nosocomial infections
- Chapter 9: Imaging Bacteria and biofilm by Field Emission Scanning Electron Microscopy (FESEM)
- Chapter 10: Probing the Surface-Attached in Vitro microbial Biofilms with Atomic Force (AFM) and Scanning probe microscopy (SPM)
- Chapter 11: Nuclear Magnetic Resonance (NMR) and Surface Plasmon Resonance (SPR) imaging as an advanced tool for examining biofilm matrix (Structure, Composition, Dynamics)
- Section VI: Molecular Biology and recombinant DNA techniques in Biofilm research
- Chapter 12: Genetic Basis of Biofilm formation and spread of nosocomial infections
- Chapter 13: Monitoring cell distribution and apoptosis in sessile forms of microbial biofilm: Flow cytometry -fluorescence activated cell sorting (FCM-FACS)
- Chapter 14: Monitoring gene expression in sessile forms of microbial biofilm: Polymerase chain reaction (PCR) and Real Time Polymerase chain reaction (RT-PCR).